Human T cell activation. III. Rapid induction of a phosphorylated 28 kD/32 kD disulfide-linked early activation antigen (EA 1) by 12-o-tetradecanoyl phorbol-13-acetate, mitogens, and antigens.
Human T cell activation. III. Rapid induction of a phosphorylated 28 kD/32 kD disulfide-linked early activation antigen (EA 1) by 12-o-tetradecanoyl phorbol-13-acetate, mitogens, and antigens.
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DOI：
10.1084/jem.164.6.1988
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发表时间：
1986-12-01
期刊：
影响因子：
--
通讯作者：
Fu SM
中科院分区：
文献类型：
--
作者：
Hara T;Jung LK;Bjorndahl JM;Fu SM
以12-O-十四酰佛波醇-13-乙酸酯(TPA)激活的人T细胞为免疫原，制备了一种针对60-kD蛋白的单抗--早期激活抗原1(EA-1)，该蛋白含有28-kD和32-kD亚基。这两个亚基都被磷酸化了。用间接免疫荧光法测定，在大约60%的分离和冷冻胸腺细胞上可以很容易地检测到EA-1抗原。在6-7%的血淋巴细胞上可检测到低水平的EA-1表达。TPA活化的T细胞在活化后30min即可表达EA-1。培养1h，85-90%的T细胞表达EA-1；3-4h，95%以上的T细胞表达EA-1。虽然EA 1+T细胞的百分率没有变化，但染色强度略有增加。18~2 4h后，EA1+细胞百分率和染色强度逐渐降低。TPA诱导的EA-1表达不依赖于单核细胞。未经玫瑰花环选择分离的T细胞经TPA处理后，EA 1的表达略有延迟。在单核细胞比例为1~2%的T细胞群中，约有50~90%的T细胞在PHA或ConA激活后表达EA-1，其动态变化较慢。在这些激活过程中，EA 1的表达先于IL-2-R的表达。同样，在MLR中被可溶性抗原(破伤风类毒素和PPD)和同种异体抗原激活的T细胞在较长时间的孵育后也表达EA 1。第6天，约20%的T细胞表达EA-1。EA-1的表达并不局限于活化的T细胞。经TPA或抗IgM抗体联合B细胞生长因子激活的B细胞表达EA-1。EA-1表达动力学明显减慢，染色强度减弱。反复尝试检测静息和TPA激活的单核细胞和粒细胞上的EA 1均未成功。然而，在非淋巴样细胞系中检测到EA-1表明EA-1可能具有更广泛的细胞分布。EA-1的表达是由于EA-1的从头合成，因为EA-1的诱导被放线菌素D和放线菌素D阻断。
With human T cells activated by 12-o-tetradecanoyl phorbol-13-acetate (TPA) as immunogen, an IgG2a mAb, early activation antigen 1 (EA 1), was generated against a 60-kD protein with disulfide-linked 28-kD and 32-kD subunits. Both subunits were phosphorylated. The antigen, EA 1, was readily detected on approximately 60% of isolated and cryopreserved thymocytes, as determined by indirect immunofluorescence. A low level of EA 1 expression was detectable on 6-7% of blood lymphocytes. TPA- activated T cells expressed EA 1 as early as 30 min after activation. By 1 h, 85-90% of the T cells stained with mAb EA 1. By 3-4 h, the expression of EA 1 was detected in greater than 95% of the T cells. Although the percentages of EA 1+ T cells did not change, the intensity of staining increased slightly. After 18-24 h, both the percentage of EA 1+ cells and the intensity of staining decreased gradually. TPA- induced EA 1 expression was independent of monocytes. EA 1 expression was slightly delayed in T cells that were isolated without the rosette selection and treated with TPA. Nevertheless, greater than 85% of these T cells expressed EA 1 within 1 h, and the maximal number of EA 1+ T cells was also detected at 3-4 h. In T cell populations with 1-2% monocytes, about 50-90% of the PHA- or Con A-activated T cells expressed EA 1 with a slower kinetics. EA 1 expression preceded that of IL-2-R in these activation processes. Similarly, T cells activated by soluble antigens (tetanus toxoid and PPD) and alloantigens in MLR also expressed EA 1 after a longer incubation. Approximately 20% of the T cells stained for EA 1 at day 6. EA 1 expression was not limited to activated T cells. B cells activated by TPA or anti-IgM antibody plus B cell growth factor expressed EA 1. The kinetics of EA 1 expression was markedly slower and the staining was less intense. Repeated attempts to detect EA 1 on resting and TPA-activated monocytes and granulocytes have not been successful. However, the detection of EA 1 in nonlymphoid cell lines would indicate that EA 1 may have a broader cell distribution. EA 1 expression was due to de novo synthesis, as the induction of EA 1 was blocked by cycloheximide and actinomycin D.(ABSTRACT TRUNCATED AT 400 WORDS)
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影响因子：
15.3
作者：
HOLTER, W;FISCHER, GF;KNAPP, W
通讯作者：
KNAPP, W
DOI：
10.1016/0090-1229(80)90042-2
发表时间：
1980-01-01
期刊：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
影响因子：
--
作者：
CHIORAZZI, N;FU, SM;KUNKEL, HG
通讯作者：
KUNKEL, HG
DOI：
10.1084/jem.161.6.1513
发表时间：
1985-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hara T;Fu SM;Hansen JA
通讯作者：
Hansen JA
影响因子：
2.7
作者：
HERCEND, T;RITZ, J;REINHERZ, EL
通讯作者：
REINHERZ, EL
影响因子：
15.3
作者：
DOWER, SK;KRONHEIM, SR;URDAL, DL
通讯作者：
URDAL, DL