Cigarette smoking is associated with an altered vaginal tract metabolomic profile.
Cigarette smoking is associated with an altered vaginal tract metabolomic profile.
复制标题
DOI：
10.1038/s41598-017-14943-3
复制
发表时间：
2018-01-16
影响因子：
4.6
通讯作者：
Brotman RM
中科院分区：
文献类型：
--
作者：
Nelson TM;Borgogna JC;Michalek RD;Roberts DW;Rath JM;Glover ED;Ravel J;Shardell MD;Yeoman CJ;Brotman RM
吸烟与细菌性阴道病 (BV) 的诊断和缺乏保护性乳杆菌属的阴道微生物群有关。由于吸烟与阴道微生物群和 BV 之间的联系机制尚不清楚，我们试图比较吸烟者和非吸烟者（17 名吸烟者/19 名非吸烟者）的阴道代谢组。在横断面研究中通过气相和液相色谱质谱法测定代谢组学特征。对 16S rRNA 基因群体的分析显示，样本分为三种群落状态类型 (CST)：CST-I（卷曲乳杆菌为主）、CST-III（L. iners 为主）或 CST-IV（低乳酸菌）。我们鉴定了 607 种代谢物，其中 12 种代谢物在吸烟者和非吸烟者之间存在显着差异（q 值 < 0.05）。正如预期的那样，吸烟者的尼古丁以及分解代谢物可替宁和羟基可替宁显着较高。在分类为 CST-IV 的女性中，吸烟者体内的生物胺（包括胍丁胺、尸胺、腐胺、色胺和酪胺）含量显着较高，而二肽含量则较低。已知这些生物胺会影响感染性病原体的毒力并导致阴道恶臭。我们的数据表明，吸烟与重要的阴道代谢物的差异有关，吸烟的女性，尤其是乳酸杆菌数量较少的女性，可能会增加对泌尿生殖系统感染的敏感性并增加恶臭。
Cigarette smoking has been associated with both the diagnosis of bacterial vaginosis (BV) and a vaginal microbiota lacking protective Lactobacillus spp. As the mechanism linking smoking with vaginal microbiota and BV is unclear, we sought to compare the vaginal metabolomes of smokers and non-smokers (17 smokers/19 non-smokers). Metabolomic profiles were determined by gas and liquid chromatography mass spectrometry in a cross-sectional study. Analysis of the 16S rRNA gene populations revealed samples clustered into three community state types (CSTs) ---- CST-I (L. crispatus-dominated), CST-III (L. iners-dominated) or CST-IV (low-Lactobacillus). We identified 607 metabolites, including 12 that differed significantly (q-value < 0.05) between smokers and non-smokers. Nicotine, and the breakdown metabolites cotinine and hydroxycotinine were substantially higher in smokers, as expected. Among women categorized to CST-IV, biogenic amines, including agmatine, cadaverine, putrescine, tryptamine and tyramine were substantially higher in smokers, while dipeptides were lower in smokers. These biogenic amines are known to affect the virulence of infective pathogens and contribute to vaginal malodor. Our data suggest that cigarette smoking is associated with differences in important vaginal metabolites, and women who smoke, and particularly women who are also depauperate for Lactobacillus spp., may have increased susceptibilities to urogenital infections and increased malodor.
登录
查看更多内容
影响因子：
3.1
作者：
Balkus JE;Richardson BA;Rabe LK;Taha TE;Mgodi N;Kasaro MP;Ramjee G;Hoffman IF;Abdool Karim SS
通讯作者：
Abdool Karim SS
DOI：
10.1086/657320
发表时间：
2010-12-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brotman RM;Klebanoff MA;Nansel TR;Yu KF;Andrews WW;Zhang J;Schwebke JR
通讯作者：
Schwebke JR
影响因子：
9.4
作者：
BRISELDEN, AM;MONCLA, BJ;HILLIER, SL
通讯作者：
HILLIER, SL
影响因子：
11.8
作者：
Cherpes, TL;Meyn, LA;Hillier, SL
通讯作者：
Hillier, SL
DOI：
10.1155/s1064744901000060
发表时间：
2001-01-01
影响因子：
--
作者：
Aroutcheva, A A;Simoes, J A;Faro, S
通讯作者：
Faro, S