A Mouse Model of Endometriosis with Nanoparticle Labeling for In Vivo Photoacoustic Imaging.
A Mouse Model of Endometriosis with Nanoparticle Labeling for In Vivo Photoacoustic Imaging.
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DOI：
10.1007/s43032-022-00980-5
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发表时间：
2022-10
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--
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中科院分区：
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子宫内膜异位症是女性生殖道的一种疾病，其特征是子宫外长出了子宫内膜样组织。虽然它是盆腔疼痛和不孕症的常见原因，但目前还没有可靠的非侵入性方法来非手术诊断子宫内膜异位症的存在，导致症状发生的病理生理机制还需要进一步探讨。由于患者的异质性和延迟的诊断，通常使用动物模型来研究子宫内膜异位症的发生发展，但由于需要在多个终点测试各种治疗方法和实验条件的大量动物，这些动物模型的成本很高。在这里，我们描述了一种通过诱导纳米颗粒标记的小鼠子宫内膜异位症样病变来合成具有临床前应用的多模式成像异硫氰酸金荧光素(FITC)纳米颗粒的方法。在受体诱发子宫内膜异位症之前，用金-FITC纳米颗粒标记供体子宫内膜组织片段，可以用光声成像在体内检测金标记的病变。同样的成像方法也可以用来非侵入性地观察怀孕小鼠的胚胎。此外，金纳米颗粒上的偶联FITC染料允许在荧光解剖显微镜下轻松分离标记的病变组织。解剖后，金-FITC纳米颗粒的存在和病变的子宫内膜样组织学可以通过荧光成像、金增强和免疫染色来证实。这种子宫内膜异位症样病变的活体成像和荧光引导解剖的方法将为子宫内膜异位症的发展和进展以及子宫内膜异位症相关性不孕症的纵向研究提供新的实验可能性。
Endometriosis is a condition of the female reproductive tract characterized by endometrium-like tissue growing outside the uterus. Though it is a common cause of pelvic pain and infertility, there is currently no reliable noninvasive method to diagnose the presence of endometriosis without surgery, and the pathophysiological mechanisms that lead to the occurrence of symptoms require further inquiry. Due to patient heterogeneity and delayed diagnosis, animal models are commonly used to study the development of endometriosis, but these are costly due to the large number of animals needed to test various treatments and experimental conditions at multiple endpoints. Here, we describe a method for synthesis of multimodal imaging gold-fluorescein isothiocyanate (FITC) nanoparticles with preclinical application via induction of nanoparticle-labeled endometriosis-like lesions in mice. Labeling donor endometrial tissue fragments with gold-FITC nanoparticles prior to induction of endometriosis in recipients enables in vivo detection of the gold-labeled lesions with photoacoustic imaging. The same imaging method can be used to visualize embryos noninvasively in pregnant mice. Furthermore, the conjugated FITC dye on the gold nanoparticles allows easy isolation of labeled lesion tissue under a fluorescence dissection microscope. After dissection, the presence of gold-FITC nanoparticles and endometrium-like histology of lesions can be verified through fluorescence imaging, gold enhancement, and immunostaining. This method for in vivo imaging of endometriosis-like lesions and fluorescence-guided dissection will permit new experimental possibilities for the longitudinal study of endometriosis development and progression as well as endometriosis-related infertility.
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影响因子：
3.7
作者：
Bilotas MA;Olivares CN;Ricci AG;Baston JI;Bengochea TS;Meresman GF;Barañao RI
通讯作者：
Barañao RI
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6
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影响因子：
6
作者：
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通讯作者：
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影响因子：
2.8
作者：
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通讯作者：
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