The effect of subacute denervation on the electrical anisotropy of skeletal muscle: implications for clinical diagnostic testing.
The effect of subacute denervation on the electrical anisotropy of skeletal muscle: implications for clinical diagnostic testing.
复制标题
DOI：
10.1016/j.clinph.2010.01.017
复制
发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Rutkove SB
中科院分区：
文献类型：
--
作者：
Ahad MA;Narayanaswami P;Kasselman LJ;Rutkove SB
施加的电流优先沿着而不是穿过肌肉纤维流动，这种特性称为各向异性。在这项研究中，我们研究了去神经后肌肉各向异性的变化。8只成年雄性大鼠行坐骨神经夹伤，1 ~ 2.5周后取腓肠肌。还获得了另外12只健康大鼠的肌肉。对组织及其电导率和相对介电常数（即，其极化率）计算。通过计算电导率和介电常数差异，从纵向值中减去横向值，确定组织的各向异性。还进行了肌纤维和血管定量。在整个频谱上，坐骨神经挤压动物的平均电导率差异高于（p <0.05）健康动物，这是由于纵向电导率的增加大于横向电导率。例如，在10 kHz下，健康动物的电导率差异为0.15 S/m，挤压后动物的电导率差异为0.29 S/m。相对介电常数差值，但是，组间相似。有一个很强的相关性的电导率各向异性肌纤维的大小，但不血管面积。亚急性失神经损伤后，肌肉电导率各向异性显著增加。这种各向异性的改变与电阻抗肌电描记术的临床应用直接相关。我们还推测，它可能会影响其他形式的诊断测试，包括针肌电图和磁共振成像。
Applied electrical current flows preferentially along rather than across muscle fibers, a characteristic called anisotropy. In this study, we investigate the alteration in muscle anisotropy after denervation. Eight adult male rats underwent sciatic nerve crush and the gastrocnemius was harvested from 1 to 2.5 weeks later. Muscle from 12 additional healthy rats was also obtained. Multifrequency electrical impedance measurements were made on the tissue and its conductivity and relative permittivity (i.e., its polarizability) calculated. Anisotropy of the tissue was determined by calculating conductivity and permittivity differences, subtracting transverse from longitudinal values. Muscle fiber and blood vessel quantification were also performed. The mean conductivity difference for sciatic crush animals was higher (p <0.05) than for the healthy animals across the frequency spectrum, due to a greater increase in longitudinal conductivity than in transverse conductivity. For example, at 10 kHz, the conductivity difference was 0.15 S/m for healthy animals and 0.29 S/m for post-crush animals. Relative permittivity difference values, however, were similar between groups. There was a strong correlation of conductivity anisotropy to muscle fiber size but not to blood vessel area. Anisotropy of muscle conductivity increases markedly after subacute denervation injury. This alteration in anisotropy has direct relevance to the clinical application of electrical impedance myography. We also speculate that it may impact other forms of diagnostic testing, including needle electromyography and magnetic resonance imaging.
登录
查看更多内容
影响因子：
3.5
作者：
Aaron, R;Huang, M;Shiffman, CA
通讯作者：
Shiffman, CA
影响因子：
20.1
作者：
RUSH, S;MCFEE, R;ABILDSKOV, JA
通讯作者：
ABILDSKOV, JA
影响因子：
5.3
作者：
Wessig, C;Koltzenburg, M;Bendszus, M
通讯作者：
Bendszus, M
影响因子：
3.2
作者：
Ahad, Mohammad A.;Fogerson, P. Michelle;Rutkove, Seward B.
通讯作者：
Rutkove, Seward B.
影响因子：
4.7
作者：
Nie, Rui;Sunmonu, N. Abimbola;Rutkove, Seward B.
通讯作者：
Rutkove, Seward B.