Differentiation of feline coronavirus type I and II infections by virus neutralization test.
Differentiation of feline coronavirus type I and II infections by virus neutralization test.
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DOI：
10.1016/j.vetmic.2007.04.031
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发表时间：
2007-10-06
影响因子：
3.3
通讯作者：
Iwata H
中科院分区：
文献类型：
--
作者：
Shiba N;Maeda K;Kato H;Mochizuki M;Iwata H
猫冠状病毒（FCoV）根据其体外生长和抗原性分为I型和II型。本研究应用病毒中和试验（VN）对FCoV感染进行分型。临床和血清学诊断为猫传染性腹膜炎（FIP）的猫的血清具有显着较高的VN滴度，以I型FCoV，和实验感染FIPV II型的猫的血清具有高VN滴度，以II型，但不是I型病毒。2004 - 2005年共采集79份猫血清，采用VN试验评价血清阳性率，结果如下：（1）50只猫FCoV阳性率为63.3%;（2）在50份FCoV阳性猫血清样品中，49份（98%）显示出对I型病毒的显著较高滴度，仅1份（2%）显示出对II型病毒的显著较高滴度。这些结果表明，本文所述的VN试验可用于猫的FCoV感染的血清学鉴别，并且FCoV I型是近年来日本的优势型。
Feline coronavirus (FCoV) is divided into two types I and II, based on their growth in vitro and antigenicity. In this study, virus neutralization (VN) test was applied for type differentiation of FCoV infections. Sera of cats which were clinically and serologically diagnosed as feline infectious peritonitis (FIP) possessed significantly higher VN titers to type I FCoV, and sera from cats experimentally infected with FIPV type II had high VN titers to type II but not type I viruses. A total of 79 cat sera collected in the years between 2004 and 2005 were examined to evaluate seroprevalence by the VN test, showing the following results: (1) 50 cats (63.3%) were sero-positive to FCoV; (2) of the 50 FCoV positive cat serum samples, 49 (98%) showed significantly higher titers to type I virus and only one (2%) for type II virus. These results indicate that the VN test described here can be used for serological differentiation of FCoV infections of cats, and that FCoV type I is a dominant type in recent years of Japan.
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影响因子：
1.2
作者：
HOHDATSU, T;OKADA, S;KOYAMA, H
通讯作者：
KOYAMA, H
影响因子：
3.3
作者：
Vennema, H
通讯作者：
Vennema, H
影响因子：
3.3
作者：
Hohdatsu T;Sasamoto T;Okada S;Koyama H
通讯作者：
Koyama H
影响因子：
5.4
作者：
CORAPI, WV;OLSEN, CW;SCOTT, FW
通讯作者：
SCOTT, FW
DOI：
10.1128/cdli.12.10.1209-1215.2005
发表时间：
2005-10-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Kummrow, M;Meli, ML;Lutz, H
通讯作者：
Lutz, H