A new allele of flower color gene W1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean Glycine soja.
A new allele of flower color gene W1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean Glycine soja.
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DOI：
10.1186/1471-2229-10-155
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发表时间：
2010-07-28
影响因子：
5.3
通讯作者：
Iwashina T
中科院分区：
文献类型：
--
作者：
Takahashi R;Dubouzet JG;Matsumura H;Yasuda K;Iwashina T
大豆是大豆的野生亲戚，开紫色的花。野生大豆的花色变异体在自然生境中尚未发现。B 09121是在日本南部发现的一个具有浅紫色花的品种。遗传分析表明，导致浅紫色花的基因与编码类黄酮3 '5'-羟化酶（F3 '5'H）的W1基因座是等位的。将新的等位基因命名为w1-lp。该位点的显性关系为W1 >w1-lp >w1。B 09121与含有w1等位基因的Clark近等基因系杂交，获得1株紫花F2代植株和4株紫花F3代植株。B 09121的花瓣含有较低量的四种主要花青素（锦葵色素3，5-二-O-葡萄糖苷、矮牵牛色素3，5-二-O-葡萄糖苷、飞燕草色素3，5-二-O-葡萄糖苷和飞燕草色素3-O-葡萄糖苷），它们在紫色花中常见，并含有少量的上述花青素、芍药色素3，5-二-O-葡萄糖苷、矢车菊色素3，5-二-O-葡萄糖苷和矢车菊素3-O-葡萄糖苷，表明F3 '5'H活性降低，类黄酮3 '-羟化酶活性增加。通过RT-PCR从Clark和B 09121中克隆F3 '5'H cDNA。B 09121的cDNA具有独特的碱基替换，导致在氨基酸位置210处缬氨酸被甲硫氨酸替换。通过dCAPS分析确定碱基取代。在F2群体中，与dCAPS标记相关的多态性与花色共分离。F_3后代检测、dCAPS和indel分析表明，紫色花植株可能是由于基因内重组，导致基因功能障碍的65 bp插入片段可能已被消除。B 09121可能是自然界中发现的第一个花色变异的例子。浅紫色花由W1基因座上一个新的等位基因控制，该基因编码F3 '5'H。花瓣中含有大豆和G.大豆。B 09121为研究F3 '5'H基因的结构和功能特性以及花色在G.大豆到自然栖息地。
Glycine soja is a wild relative of soybean that has purple flowers. No flower color variant of Glycine soja has been found in the natural habitat. B09121, an accession with light purple flowers, was discovered in southern Japan. Genetic analysis revealed that the gene responsible for the light purple flowers was allelic to the W1 locus encoding flavonoid 3'5'-hydroxylase (F3'5'H). The new allele was designated as w1-lp. The dominance relationship of the locus was W1 >w1-lp >w1. One F2 plant and four F3 plants with purple flowers were generated in the cross between B09121 and a Clark near-isogenic line with w1 allele. Flower petals of B09121 contained lower amounts of four major anthocyanins (malvidin 3,5-di-O-glucoside, petunidin 3,5-di-O-glucoside, delphinidin 3,5-di-O-glucoside and delphinidin 3-O-glucoside) common in purple flowers and contained small amounts of the 5'-unsubstituted versions of the above anthocyanins, peonidin 3,5-di-O-glucoside, cyanidin 3,5-di-O-glucoside and cyanidin 3-O-glucoside, suggesting that F3'5'H activity was reduced and flavonoid 3'-hydroxylase activity was increased. F3'5'H cDNAs were cloned from Clark and B09121 by RT-PCR. The cDNA of B09121 had a unique base substitution resulting in the substitution of valine with methionine at amino acid position 210. The base substitution was ascertained by dCAPS analysis. The polymorphism associated with the dCAPS markers co-segregated with flower color in the F2 population. F3 progeny test, and dCAPS and indel analyses suggested that the plants with purple flowers might be due to intragenic recombination and that the 65 bp insertion responsible for gene dysfunction might have been eliminated in such plants. B09121 may be the first example of a flower color variant found in nature. The light purple flower was controlled by a new allele of the W1 locus encoding F3'5'H. The flower petals contained unique anthocyanins not found in soybean and G. soja. B09121 may be a useful tool for studies of the structural and functional properties of F3'5'H genes as well as investigations on the role of flower color in relation to adaptation of G. soja to natural habitats.
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影响因子：
3.1
作者：
Iwashina, Tsukasa;Benitez, Eduardo R.;Takahashi, Ryoji
通讯作者：
Takahashi, Ryoji
影响因子：
3.1
作者：
Iwashina, Tsukasa;Githiri, Stephen M.;Takahashi, Ryoji
通讯作者：
Takahashi, Ryoji
影响因子：
2.3
作者：
Chen, YW;Nelson, RL
通讯作者：
Nelson, RL
影响因子：
14.9
作者：
MURRAY, MG;THOMPSON, WF
通讯作者：
THOMPSON, WF
影响因子：
2.3
作者：
Zabala, Gracia;Vodkin, Lila O.
通讯作者：
Vodkin, Lila O.