Antibacterial and Cellular Response Toward a Gasotransmitter-Based Hybrid Wound Dressing.
Antibacterial and Cellular Response Toward a Gasotransmitter-Based Hybrid Wound Dressing.
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DOI：
10.1021/acsbiomaterials.9b00737
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发表时间：
2019-08-12
影响因子：
5.8
通讯作者：
Handa, Hitesh
中科院分区：
文献类型：
--
作者：
Pant, Jitendra;Pedaparthi, Shriya;Hopkins, Sean P.;Goudie, Marcus J.;Douglass, Megan E.;Handa, Hitesh
为了有效地愈合伤口，需要调节生物过程如感染、血管生成和成纤维细胞增殖和迁移。如果不这样做，可能会延迟整体伤口愈合，并增加痛苦和医疗费用。内源性一氧化氮（NO）是一种众所周知的气体递质在人类和其他哺乳动物的自然愈合过程。为了利用其固有的能力，在本研究中，外源性NO供体（S-亚硝基谷胱甘肽，GSNO）被整合到一个混合的配方组成的天然聚合物（藻酸盐）和合成聚合物（聚乙烯醇）（PVA）。藻酸盐-PVA-GSNO敷料显示出持续72小时的NO释放，分别导致99.89 ± 0.40%和98.93 ± 0.69%的金黄色葡萄球菌和铜绿假单胞菌根除，这是伤口感染的最常见病原体。与不含GSNO的对照相比，设计的敷料导致人内皮细胞增殖增加3倍，显示其血管生成潜力。此外，与对照藻酸盐-PVA相比，暴露于藻酸盐-PVA-GSNO敷料沥滤物的小鼠成纤维细胞显示出显著更高的增殖，表明成纤维细胞增殖中外源性GSNO的NO释放。与相应的对照敷料相比，使用基于GSNO的敷料时，成纤维细胞迁移速度更快，导致体外伤口模型在48小时内完全闭合。多孔敷料还具有重要的物理性质，如溶胀、水蒸气透过率和含水量，这些都是有效伤口愈合所需的。总体而言，本研究支持使用治疗性藻酸盐-PVA-GSNO敷料为加速伤口愈合提供支持性环境的可能性。
Biological processes such as infection, angiogenesis, and fibroblast proliferation and migration need to be regulated for effective healing of a wound. Failing to do so can delay the overall wound healing and add to the suffering and healthcare cost. Endogenous nitric oxide (NO) is a well-known gasotransmitter in the natural healing process in humans and other mammals. To utilize its inherent ability in the current study, an exogenous NO donor (S-nitroso-glutathione, GSNO) was integrated into a hybrid formulation consisting of a natural polymer (alginate) and a synthetic polymer (poly(vinyl alcohol) (PVA)). The alginate–PVA–GSNO dressings showed a sustained NO release for 72 h that resulted in 99.89 ± 0.40% and 98.93 ± 0.69% eradication of Staphylococcus aureus and Pseudomonas aeruginosa, respectively, which are among the most common causal agents of wound infections. The designed dressings resulted in a 3-fold increase in the proliferation of human endothelial cells when compared with control without GSNO showing its angiogenic potential. In addition, mouse fibroblast cells exposed to leachates from alginate–PVA–GSNO dressings showed significantly higher proliferation when compared to control alginate–PVA showing the NO release from exogenous GSNO in fibroblast proliferation. Fibroblast migration was shown to be much faster with GSNO-based dressings when compared to corresponding control dressings resulting in complete closure of an in vitro wound model within 48 h. The porous dressings also possessed important physical properties such as swelling, water vapor transmission, and moisture content that are desirable for effective wound healing. Overall, this study supports the possibility of using therapeutic alginate–PVA–GSNO dressing to provide a supportive environment for accelerated wound healing.
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影响因子：
9.8
作者：
Bishop, E T;Bell, G T;Wheatley, D N
通讯作者：
Wheatley, D N
影响因子：
4.9
作者：
Dykhuizen, RS;Frazer, R;Leifert, C
通讯作者：
Leifert, C
影响因子：
6
作者：
Kamoun, Elbadawy A.;Kenawy, El-Refaie S.;Eldin, Mohamed S. Mohy
通讯作者：
Eldin, Mohamed S. Mohy
DOI：
10.1080/00914037.2016.1163570
发表时间：
2016
期刊：
International journal of polymeric materials
影响因子：
--
作者：
Goudie MJ;Brisbois EJ;Pant J;Thompson A;Potkay JA;Handa H
通讯作者：
Handa H
影响因子：
5.8
作者：
Kim, Jong Oh;Park, Jung Kil;Choi, Han-Gon
通讯作者：
Choi, Han-Gon