Complete recombinant silk-elastinlike protein-based tissue scaffold.
Complete recombinant silk-elastinlike protein-based tissue scaffold.
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DOI：
10.1021/bm100469w
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发表时间：
2010-12-13
影响因子：
6.2
通讯作者：
Wu, Xiaoyi
中科院分区：
文献类型：
--
作者：
Qiu, Weiguo;Huang, Yiding;Teng, Weibing;Cohn, Celine M.;Cappello, Joseph;Wu, Xiaoyi
基于蛋白质的生物材料，无论是天然来源的还是基因工程的，由于其比合成材料更好的生物相容性和特异性，已经被广泛地制造成用于组织工程应用的纳米纤维支架。然而，其较差的机械性能通常需要使用合成聚合物来增强基于蛋白质的组织支架。在这项研究中，我们报道了一种完全重组的丝状弹性蛋白组织支架的电纺丝，该支架具有良好的力学性能和生物相容性。特别是，从天然丝素和弹性蛋白中提取的含有重复序列的SELP-47K被电纺制成纳米纤维支架，并通过化学蒸汽处理和机械预处理来稳定。当在37℃的1X PBS中完全水合时，机械预处理的SELP-47K支架的弹性模量为3.4~13.2 Mpa，极限拉伸强度为5.7~13.5 Mpa，变形能力为100~130%应变，回弹为80.6~86.9%，接近或超过蛋白质合成的混合聚合物支架。此外，SELP-47K纳米纤维支架还促进了细胞的附着和生长，显示了其体外生物相容性。
Due to their improved biocompatibility and specificity over synthetic materials, protein-based biomaterials, either derived from natural sources or genetically engineered, have been widely fabricated into nanofibrous scaffolds for tissue engineering applications. However, their inferior mechanical properties often require the reinforcement of protein-based tissue scaffolds using synthetic polymers. In this study, we report the electrospinning of a completely recombinant silk-elastinlike protein-based tissue scaffold with excellent mechanical properties and biocompatibility. In particular, SELP-47K containing tandemly repeated polypeptide sequences derived from native silk and elastin was electrospun into nanofibrous scaffolds, and stabilized via chemical vapor treatment and mechanical preconditioning. When fully hydrated in 1x PBS at 37 °C, mechanically preconditioned SELP-47K scaffolds displayed elastic moduli of 3.4 to 13.2 MPa, ultimate tensile strengths of 5.7 to 13.5 MPa, deformabilities of 100 to 130% strain, and resilience of 80.6 to 86.9%, closely matching or exceeding those of protein-synthetic blend polymeric scaffolds. Additionally, SELP-47K nanofibrous scaffolds promoted cell attachment and growth demonstrating their in vitro biocompatibility.
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影响因子：
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