High prevalence of human rhinovirus C infection in Thai children with acute lower respiratory tract disease.
High prevalence of human rhinovirus C infection in Thai children with acute lower respiratory tract disease.
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DOI：
10.1016/j.jinf.2009.05.009
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Poovorawan Y
中科院分区：
文献类型：
--
作者：
Linsuwanon P;Payungporn S;Samransamruajkit R;Posuwan N;Makkoch J;Theanboonlers A;Poovorawan Y
确定泰国患有下呼吸道疾病的儿童中人鼻病毒（HRV）感染的流行情况，并监测住院儿科患者中HRV种类与临床表现之间的关系。2006-2007年2月期间，从泰国朱拉隆功国王纪念医院住院的儿科患者中收集了289份鼻咽（NP）吸痰标本。每个样品提取核酸，然后用半巢式RT-PCR扩增VP4/2进行HRV检测。采用PCR、RT-PCR或real - time PCR检测其他呼吸道病毒病原体。利用VP4区核苷酸序列进行基因分型和系统发育树构建。总共289份标本中有87份HRV呈阳性，表明年患病率为30%。喘息或哮喘加重是感染患者最常见的临床表现。序列分析和系统进化树分析显示，29份（33%）属于HRV-A型，8份（9%）属于HRV-B型。大多数HRV阳性样本为HRV- c(58%)。雨季泰国儿童种群中以C种居多（p < 0.05）。HRV-C与其他呼吸道病毒病原体共感染的频率约为40%。HRV-C是优势种，是泰国婴幼儿急性下呼吸道感染的病原之一，是引起喘息和哮喘加重的原因。
To determine the prevalence of human rhinoviruses (HRV) infections in children with lower respiratory disease in Thailand and monitor the association between species of HRV and clinical presentation in hospitalized paediatric patients. Two hundred and eighty-nine nasopharyngeal (NP) suction specimens were collected from hospitalized paediatric patients admitted to King Chulalongkorn Memorial Hospital, Thailand during February 2006–2007. Nucleic acids were extracted from each sample with subsequent amplification of VP4/2 by semi-nested RT-PCR for HRV detection. Other viral respiratory pathogens were also detected by PCR, RT-PCR or real time PCR. Nucleotide sequences of the VP4 region were used for genotyping and phylogenetic tree construction. In total, 87 of 289 specimens were positive for HRV indicating an annual prevalence of 30%. Wheezing or asthma exacerbation was the most common clinical presentation observed in infected patients. Sequence analysis and phylogenetic tree showed that 29 (33%) and 8 (9%) specimens belonged to HRV-A and HRV-B, respectively. Most of the HRV positive samples were HRV-C (58%). Moreover, species C was predominantly found in the paediatric population of Thailand in raining season (p < 0.05). The frequency of co-infection of HRV-C with other respiratory viral pathogens was approximately 40%. HRV-C represents the predominant species and is one of the etiologic agents in acute lower respiratory tract infection, causes of wheezing and asthma exacerbation in infants and young children in Thailand.
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DOI：
10.1016/j.jaci.2008.10.007
发表时间：
2009-01
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Miller EK;Edwards KM;Weinberg GA;Iwane MK;Griffin MR;Hall CB;Zhu Y;Szilagyi PG;Morin LL;Heil LH;Lu X;Williams JV;New Vaccine Surveillance Network
通讯作者：
New Vaccine Surveillance Network
影响因子：
28.2
作者：
Samransamruajkit, R;Thanasugarn, W;Poovorawan, Y
通讯作者：
Poovorawan, Y
影响因子：
4.4
作者：
Chantzi, F. M.;Papadopoulos, N. G.;Kafetzis, D. A.
通讯作者：
Kafetzis, D. A.
影响因子：
11.8
作者：
Khetsuriani N;Lu X;Teague WG;Kazerouni N;Anderson LJ;Erdman DD
通讯作者：
Erdman DD
影响因子：
12.7
作者：
De Silva, D;Dagher, H;Bardin, PG
通讯作者：
Bardin, PG