Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study.
Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study.
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DOI：
10.1111/j.1600-0765.2008.01119.x
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发表时间：
2009-06
影响因子：
3.5
通讯作者：
Haffajee AD
中科院分区：
文献类型：
--
作者：
Teles RP;Likhari V;Socransky SS;Haffajee AD
唾液已被提出作为一种非侵入性诊断流体，可用于口腔和全身性疾病的诊断。唾液生物标志物（例如细胞因子）的水平可能可以用作区分牙周健康与牙周炎受试者的替代品。因此，本研究的目的是确定唾液中10种细胞因子的水平是否在牙周健康和牙周炎受试者之间存在差异。这10种细胞因子的浓度和牙周病的临床参数之间的相关性也进行了研究。在这项横断面研究中，对74名慢性牙周炎患者和44名牙周健康者进行了牙周检查，并使用多重微珠免疫测定法（Luminex）测定了全唾液中GM-CSF、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IFN-γ和TNF-α的水平。使用调整年龄和吸烟状态的非参数ANCOVA确定组间唾液细胞因子水平的统计学差异的显著性。使用斯皮尔曼等级相关系数来探索唾液细胞因子的平均水平与平均临床参数之间的关联。对于任何细胞因子，组间无统计学显著差异。唾液IL-8与PD（rs=0.2，p<0.05）和BOP（rs = 0.2，p<0.05）之间存在弱统计学显著正相关，唾液IL-10与AL（rs=-0.2，p<0.05）和BOP（rs=-0.3，p<0.001）之间存在弱负相关。唾液中GM-CSF、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IFN-γ和TNF-α的平均水平不能区分牙周健康和疾病。
Saliva has been proposed as a non-invasive diagnostic fluid that could be used in the diagnosis of oral and systemic diseases. The levels of salivary biomarkers such as cytokines could potentially be used as a surrogate to distinguish periodontally healthy from periodontitis subjects. Therefore, the goal of the present investigation was to determine if the levels of 10 cytokines in saliva would differ between a group of periodontally healthy and periodontitis subjects. Correlations between the concentration of these 10 cytokines and clinical parameters of periodontal disease were also examined. In this cross-sectional study, 74 chronic periodontitis and 44 periodontally healthy individuals were periodontally examined and had the levels of GM-CSF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-γ and TNF-α measured in whole saliva using a multiplexed bead immunoassay (Luminex). Significance of statistical differences in the levels of salivary cytokines between groups was determined using non-parametric ANCOVA adjusting for age and smoking status. The Spearman rank correlation coefficient was used to explore associations between mean levels of salivary cytokines and mean clinical parameters. There were no statistically significant differences between groups for any of the cytokines. There were weak statistically significant positive associations between salivary IL-8 and PD (rs=0.2, p<0.05) and BOP (rs=0.2, p<0.05) and weak negative correlations between salivary IL-10 and AL (rs=−0.2, p<0.05) and BOP (rs=−0.3, p<0.001). Mean salivary levels of GM-CSF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-γ and TNF-α could not discriminate between periodontal health and disease.
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影响因子：
6.7
作者：
Holmlund, A;Hänström, L;Lerner, UH
通讯作者：
Lerner, UH
影响因子：
6.7
作者：
REINHARDT, RA;MASADA, MP;ALLISON, AC
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ALLISON, AC
影响因子：
7.6
作者：
Sandros, J;Karlsson, C;Papapanou, PN
通讯作者：
Papapanou, PN
影响因子：
3.5
作者：
Ishihara, Y;Nishihara, T;Noguchi, T
通讯作者：
Noguchi, T
影响因子：
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作者：
Taba, Mario Jr;Kinney, Janet;Giannobile, William V
通讯作者：
Giannobile, William V