Protection of rats from adjuvant arthritis by immunization with naked DNA encoding for mycobacterial heat shock protein 65.
Protection of rats from adjuvant arthritis by immunization with naked DNA encoding for mycobacterial heat shock protein 65.
复制标题
用编码分枝杆菌热休克蛋白 65 的裸露 DNA 进行免疫，可保护大鼠免受佐剂性关节炎的影响。
作者：
S. Ragno;M. Colston;D. Lowrie;V. Winrow;D. Blake;R. Tascon
客观的
OBJECTIVE
To assess the feasibility of vaccination with naked DNA encoding for mycobacterial heat shock protein 65 (hsp65) in the modulation of experimental arthritis.
METHODS
Adjuvant arthritis (AA) was induced in Lewis rats preimmunized, intramuscularly, with a plasmid encoding for hsp65 (pCMV3.65). Clinical scores were recorded for 3-4 weeks, and histologic and radiologic parameters were evaluated. Cellular and antibody reactivity to hsp65 was assessed. The expression of the hsp65 gene was investigated in injected muscles.
RESULTS
The pCMV3.65-treated rats were significantly protected from disease development in comparison with the control groups. This finding correlated with the results of histologic and radiologic examinations of the involved joints. The message for hsp65 was detected in injected muscles. T cell proliferation and antibodies to this protein were found to be elevated in pCMV3.65-treated rats when compared with both the arthritic control (AA-induced) and the naive (did not receive adjuvant) animals.
CONCLUSION
We have demonstrated for the first time that naked DNA delivery is feasible in controlling experimental autoimmune disease. Although the actual mechanism of protection has not been fully elucidated, this simple and versatile approach could represent a useful tool in dissecting basic autoimmune mechanisms.
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由对结核分枝杆菌和软骨蛋白聚糖有反应的 T 淋巴细胞克隆诱导的关节炎。
DOI：
10.1073/pnas.82.15.5117
发表时间：
1985
影响因子：
11.1
作者：
vanEden,W;Holoshitz,J;Nevo,Z;Frenkel,A;Klajman,A;Cohen,IR
通讯作者：
Cohen,IR
DOI：
10.1073/pnas.90.24.11478
发表时间：
1993-12-15
影响因子：
11.1
作者：
FYNAN, EF;WEBSTER, RG;ROBINSON, HL
通讯作者：
ROBINSON, HL
T 淋巴细胞克隆阐明了发病机制并影响实验性关节炎的治疗。
影响因子：
--
作者：
Cohen,IR;Holoshitz,J;vanEden,W;Frenkel,A
通讯作者：
Frenkel,A
DOI：
10.1073/pnas.83.18.7013
发表时间：
1986-09-01
影响因子：
11.1
作者：
MEHRA, V;SWEETSER, D;YOUNG, RA
通讯作者：
YOUNG, RA
影响因子：
3.7
作者：
XIANG, ZQ;SPITALNIK, S;ERTL, HCJ
通讯作者：
ERTL, HCJ