Unravelling Plant Responses to Stress-The Importance of Targeted and Untargeted Metabolomics.
Unravelling Plant Responses to Stress-The Importance of Targeted and Untargeted Metabolomics.
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DOI：
10.3390/metabo11080558
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发表时间：
2021-08-22
期刊：
影响因子：
4.1
通讯作者：
Pétriacq P
中科院分区：
文献类型：
--
作者：
Allwood JW;Williams A;Uthe H;van Dam NM;Mur LAJ;Grant MR;Pétriacq P
气候变化和人口增长对环境可持续的营养食品生产提出了巨大的全球挑战。通过育种实现的作物产量提高正在减少，而农业集约化受到新出现、重新出现和地方性病虫害和病原体的限制，这些病虫害和病原体约占全球作物损失的 30%，而且气候变化导致的非生物胁迫压力也日益增加。代谢组学方法此前已为我们在分子植物病理学和植物与昆虫相互作用领域的知识做出了贡献。然而，由于代谢物挥发性和极性的巨大多样性、病原体和植物细胞的异质混合物以及代谢物周转速度快，这些目标仍然极具挑战性。揭示植物对各种个体和组合胁迫的系统生化反应，涉及监测信号化合物、第二信使、植物激素以及防御和保护性化学物质。这需要靶向和非靶向代谢组学方法，以及一系列酶测定、蛋白质测定以及蛋白质组和转录组技术。在这篇综述中，我们重点关注测量与植物胁迫相关的代谢组的技术和生物学挑战。我们通过细菌和真菌分子病理学、植物与昆虫相互作用以及环境中的非生物和组合胁迫等相关例子来说明这些挑战。我们还从关键创新代谢组学技术及其在抗逆育种中的应用的角度讨论了未来的前景。
Climate change and an increasing population, present a massive global challenge with respect to environmentally sustainable nutritious food production. Crop yield enhancements, through breeding, are decreasing, whilst agricultural intensification is constrained by emerging, re-emerging, and endemic pests and pathogens, accounting for ~30% of global crop losses, as well as mounting abiotic stress pressures, due to climate change. Metabolomics approaches have previously contributed to our knowledge within the fields of molecular plant pathology and plant–insect interactions. However, these remain incredibly challenging targets, due to the vast diversity in metabolite volatility and polarity, heterogeneous mixtures of pathogen and plant cells, as well as rapid rates of metabolite turn-over. Unravelling the systematic biochemical responses of plants to various individual and combined stresses, involves monitoring signaling compounds, secondary messengers, phytohormones, and defensive and protective chemicals. This demands both targeted and untargeted metabolomics approaches, as well as a range of enzymatic assays, protein assays, and proteomic and transcriptomic technologies. In this review, we focus upon the technical and biological challenges of measuring the metabolome associated with plant stress. We illustrate the challenges, with relevant examples from bacterial and fungal molecular pathologies, plant–insect interactions, and abiotic and combined stress in the environment. We also discuss future prospects from both the perspective of key innovative metabolomic technologies and their deployment in breeding for stress resistance.
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影响因子：
7.2
作者：
Allwood, JW;Ellis, DI;Mur, LAJ
通讯作者：
Mur, LAJ
DOI：
10.1007/978-1-61779-594-7_3
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Allwood, J William;Heald, Jim;Mur, Luis A J
通讯作者：
Mur, Luis A J
影响因子：
5.3
作者：
Ali MB;McNear DH Jr
通讯作者：
McNear DH Jr
影响因子：
4.1
作者：
Rubio B;Fernandez O;Cosson P;Berton T;Caballero M;Lion R;Roux F;Bergelson J;Gibon Y;Schurdi-Levraud V
通讯作者：
Schurdi-Levraud V
影响因子：
3.8
作者：
Baaij, B. Martina;Kim, Hye Kyong;de Jong, Tom J.
通讯作者：
de Jong, Tom J.