Clear cell injury associated with reduced expression of carbonic anhydrase II in eccrine glands consistently occurs in patients with acquired idiopathic generalized anhidrosis
Clear cell injury associated with reduced expression of carbonic anhydrase II in eccrine glands consistently occurs in patients with acquired idiopathic generalized anhidrosis
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与小汗腺碳酸酐酶 II 表达减少相关的透明细胞损伤始终发生在获得性特发性全身性无汗症患者中
DOI：
10.1111/1346-8138.15722
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发表时间：
2021
期刊：
影响因子：
3.1
通讯作者：
R Okuyama
中科院分区：
文献类型：
--
作者：
K Sano ;M Asahina ;T Uehara ;K Matsumoto ;N Araki ;R Okuyama
获得性特发性全身性无汗症（AIGA）的特点是无汗/少汗，没有其他自主神经和神经功能障碍。据认为，AIGA 患者的小汗腺分泌部分（由透明细胞、深色细胞和肌上皮细胞组成）通常不存在明显的形态学改变。然而，我们最近通过免疫组织化学揭示了 AIGA 患者小汗腺的形态学损伤。此外，据报道，抗癫痫剂托吡酯对碳酸酐酶 II (CA II) 的抑制性副作用会导致类似 AIGA 的热不耐受。为了确定 AIGA 患者小汗腺的精确形态变化和 CA II 表达，对每位临床诊断为 AIGA 的患者的无汗（无汗）和正常无汗（出汗）部位的皮肤进行了电子显微镜观察和免疫组织化学分析。我们发现 AIGA 患者无汗皮肤样本的小汗腺存在一致的透明细胞损伤。电子显微照片显示几乎所有 AIGA 患者的非出汗区域的小汗腺透明细胞出现水肿、肿胀和破坏性损伤。免疫组织化学显示，透明细胞显示 CA II 表达减少，且在无汗皮肤中分布不均匀。尽管保留了暗细胞，但一些区域显示 CA II 表达几乎完全丧失，而其他区域则显示轻度或中度丧失。选择性破坏透明细胞导致 AIGA 患者异质性萎缩对于阐明其病因可能很重要。
Acquired idiopathic generalized anhidrosis (AIGA) is characterized by anhidrosis/hypohidrosis without other autonomic and neurological dysfunctions. It has been believed that AIGA patients usually present no significant morphological alterations in the secretory portion of eccrine glands consisting of clear, dark and myoepithelial cells. However, we have recently revealed morphological damage of eccrine glands in AIGA patients by immunohistochemistry. Moreover, inhibitory side‐effects against carbonic anhydrase II (CA II) by the antiepileptic reagent topiramate have been reported to cause heat intolerance mimicking AIGA. To determine the precise morphological changes and CA II expression in eccrine glands of AIGA patients, electron microscopic observation and immunohistochemistry were applied to skin of both anhidrotic (non‐sweating) and normohidrotic (sweating‐preserved) sites, taken from each patient clinically diagnosed with AIGA. We found consistent clear cell injury in eccrine glands in anhidrotic skin samples of AIGA patients. Electron micrographs demonstrated edematous, swollen and destructive damage in clear cells of eccrine glands from non‐sweating areas of almost all AIGA patients. Immunohistochemically, clear cells showed reduced CA II expression that was heterogeneously distributed in non‐sweating skin. Some areas showed almost complete loss of CA II expression in spite of preserved dark cells, and others showed mild or moderate loss of it. Selective destruction of clear cells resulting in heterogenous atrophy in AIGA patients may be important to elucidate its etiology.
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影响因子：
9.1
作者：
KONSTADOULAKIS, MM;SYRIGOS, KN;GOLEMATIS, B
通讯作者：
GOLEMATIS, B
影响因子：
9
作者：
D. Holyoke;G. Reynoso;T. Chu
通讯作者：
T. Chu
人体小汗腺的电子显微镜研究。
DOI：
10.1679/aohc1950.27.81
发表时间：
1966
期刊：
Archivum histologicum Japonicum = Nihon soshikigaku kiroku
影响因子：
--
作者：
T. Ito;S. Shibasaki
通讯作者：
S. Shibasaki
影响因子：
--
作者：
Jeff M. Moore;S. Northway;N. Wells;E. Woolf;M. Buono
通讯作者：
M. Buono
白塞病中的碳酸酐酶 I 和 II 自身抗体。
DOI：
--
发表时间：
2017
期刊：
Acta Reumatológica Portuguesa
影响因子：
--
作者：
A. Menteşe;A. Alver;S. Demir;A. Sumer;Serap Ozer Yaman;M. Karkucak;Arzu Aydin Çapkin;Diler Us Altay;I. Turan
通讯作者：
I. Turan