Transcriptome Analysis of Developing Wheat Grains at Rapid Expanding Phase Reveals Dynamic Gene Expression Patterns.
Transcriptome Analysis of Developing Wheat Grains at Rapid Expanding Phase Reveals Dynamic Gene Expression Patterns.
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在快速扩展阶段发育的小麦晶粒的转录组分析揭示了动态基因表达模式。
DOI：
10.3390/biology11020281
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发表时间：
2022-02-10
期刊：
影响因子：
4.2
通讯作者：
Li A
中科院分区：
文献类型：
--
作者：
Guan J;Wang Z;Liu S;Kong X;Wang F;Sun G;Geng S;Mao L;Zhou P;Li A
了解小麦籽粒发育的调控机制对小麦改良至关重要。早期籽粒膨大具有胚胎发生和籽粒灌浆启动等关键生物学事件。这一发育阶段的RNA测序分析揭示了与细胞分裂、淀粉生物合成和激素生物合成相关的基因的动态表达。多条丰富的代谢途径表明，三联体之间的不平衡表达可能起着至关重要的作用。我们的工作揭示了小麦早期发育过程中复杂的调控机制，为今后小麦改良提供了有用的信息。谷物发育作为作物生命周期中的一个重要过程，对决定作物质量和产量至关重要。小麦籽粒膨大期是小麦籽粒形态快速变化和籽粒灌浆启动的早期过程。然而，现阶段人们对谷物发育的分子基础知之甚少。在这里，我们提供了小麦地方品种中国春授粉后0、2、4、6、8和10天小麦籽粒发育的时间序列转录组图谱。在相邻时间点之间，共发现26892个差异表达基因，包括1468个转录因子。共表达聚类分析和基因本体丰富揭示了细胞分裂和淀粉生物合成相关结构基因和转录因子的动态表达。此外，还鉴定了与激素代谢相关的关键基因的多样性、差异性和显著差异的表达趋势。此外，约30%的三联体表现出不平衡的表达模式，丰富了多个关键代谢途径中的基因。激素代谢相关基因，如YUC10(丝兰黄素单加氧酶10)、AOS2(丙二烯氧化物合成酶2)、CYP90D2(细胞色素P450 90D2)和CKX1(细胞分裂素脱氢酶1)，主要由A或D同源物贡献。本研究提供了小麦籽粒膨大前期转录调控的系统图景，有助于加深对小麦籽粒发育的认识，有助于小麦产量的改良。
Understanding the regulatory mechanism underlying grain development is essential for wheat improvement. The early grain expanding phase boasts critical biological events like embryogenesis and initiation of grain filling. RNA sequencing analysis of this developmental stage revealed dynamic expressions of genes related to cell division, starch biosynthesis, and hormone biosynthesis. An unbalanced expression among triads may play critical roles as shown by multiple enriched metabolic pathways. Our work demonstrated complex regulation mechanisms in early grain development and provided useful information for future wheat improvement. Grain development, as a vital process in the crop’s life cycle, is crucial for determining crop quality and yield. The wheat grain expanding phase is the early process involving the rapid morphological changes and initiation of grain filling. However, little is known about the molecular basis of grain development at this stage. Here, we provide a time-series transcriptome profile of developing wheat grain at 0, 2, 4, 6, 8, and 10 days after pollination of the wheat landrace Chinese Spring. A total of 26,892 differentially expressed genes, including 1468 transcription factors, were found between adjacent time points. Co-expression cluster analysis and Gene Ontology enrichment revealed dynamic expressions of cell division and starch biosynthesis related structural genes and transcription factors. Moreover, diverse, differential and drastically varied expression trends of the key genes related to hormone metabolism were identified. Furthermore, ~30% of triads showed unbalanced expression patterns enriching for genes in multiple pivotal metabolic pathways. Hormone metabolism related genes, such as YUC10 (YUCCA flavin-containing monooxygenase 10), AOS2 (allene oxide synthase 2), CYP90D2 (cytochrome P450 90D2), and CKX1 (cytokinin dehydrogenase 1), were dominantly contributed by A or D homoeologs of the triads. Our study provided a systematic picture of transcriptional regulation of wheat grains at the early grain expanding phase which should deepen our understanding of wheat grain development and help in wheat yield improvement.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.6
作者：
Dante RA;Larkins BA;Sabelli PA
通讯作者：
Sabelli PA
影响因子：
5.3
作者：
Liu Z;Xin M;Qin J;Peng H;Ni Z;Yao Y;Sun Q
通讯作者：
Sun Q
影响因子：
9.4
作者：
McAdam, Erin L.;Meitzel, Tobias;Ross, John J.
通讯作者：
Ross, John J.
DOI：
10.1093/plcell/koaa040
发表时间：
2021-05-05
期刊：
The Plant cell
影响因子：
--
作者：
Gao Y;An K;Guo W;Chen Y;Zhang R;Zhang X;Chang S;Rossi V;Jin F;Cao X;Xin M;Peng H;Hu Z;Guo W;Du J;Ni Z;Sun Q;Yao Y
通讯作者：
Yao Y