Peri-adolescent ethanol vapor exposure produces reductions in hippocampal volume that are correlated with deficits in prepulse inhibition of the startle.
Peri-adolescent ethanol vapor exposure produces reductions in hippocampal volume that are correlated with deficits in prepulse inhibition of the startle.
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DOI：
10.1111/acer.12125
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Crews FT
中科院分区：
文献类型：
--
作者：
Ehlers CL;Oguz I;Budin F;Wills DN;Crews FT
流行病学研究表明，过度饮酒在青少年中很普遍，并可能产生持久的神经行为后果。动物模型的使用允许从遗传背景和其他环境损害中分离青少年酒精暴露的影响。在本研究中，我们评估了青春期适度的乙醇蒸气暴露对成年期结构扩散张量成像（DTI）和行为测量的影响。在出生后第21天（PD）接收53只Wistar大鼠，从PD 23至PD 58（平均血乙醇浓度（BEC）： 169 mg%），随机分为乙醇蒸汽（14小时/10小时/天）或空气暴露35天。将动物分为两组，分别在退出乙醇蒸汽后的两个时间点处死：(1)72日龄，退出后2周；(2)退出后第128天，退出后10周。在第二组中，还评估了光/暗盒中的行为和惊吓预脉冲抑制（PPI）。每组15只动物在死后进行结构性DTI扫描。乙醇组与对照组动物体重无显著差异。体积数据显示，与72天的动物相比，128天的动物的大脑、海马、胼胝体的总体积明显大于72天的动物。与72天相比，乙醇暴露动物在128天的海马更小，心室更大，导致显著的组×时间效应。128天乙醇暴露的动物在灯箱中也有PPI下降和更多的后部，这与海马的大小显著相关。这些研究表明，Wistar大鼠海马DTI体积测量明显受到年龄和青春期前后乙醇暴露和戒断的影响。
Epidemiological studies suggest that excessive alcohol consumption is prevalent among adolescents and may have lasting neurobehavioral consequences. The use of animal models allows for the separation of the effects of adolescent ethanol exposure from genetic background and other environmental insults. In the present study the effects of moderate ethanol vapor exposure, during adolescence, on structural diffusion tensor imaging (DTI) and behavioral measures were evaluated in adulthood. A total of 53 Wistar rats were received at postnatal day (PD) 21, and were randomly assigned to ethanol vapor (14 hrs on/10 hrs off/day) or air exposure for 35 days from PD 23-PD 58 (average blood ethanol concentration (BEC): 169 mg%). Animals were received in two groups that were subsequently sacrificed at two time points following withdrawal from ethanol vapor: (1) at 72 days of age, 2 weeks following withdrawal or (2) at day 128, 10 weeks following withdrawal. In the second group, behavior in the light/dark box and prepulse inhibition of the startle (PPI) were also evaluated. Fifteen animals in each group were scanned, post mortem, for structural DTI. There were no significant differences in body weight between ethanol and control animals. Volumetric data, demonstrated that total brain, hippocampal, corpus callosum but not ventricular volume was significantly larger in the 128 day sacrificed animals as compared to the 72 day animals. The hippocampus was smaller and the ventricles larger at 128 days as compared to 72 days, in the ethanol exposed animals, leading to a significant group × time effect. Ethanol exposed animals sacrificed at 128 days also had diminished PPI and more rears in the light box that were significantly correlated with hippocampal size. These studies demonstrate that DTI volumetric measures of hippocampus are significantly impacted by age and periadolescent ethanol exposure and withdrawal in Wistar rats.
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影响因子：
3.6
作者：
Crews, Fulton Timm;Boettiger, Charlotte Ann
通讯作者：
Boettiger, Charlotte Ann
DOI：
10.1111/j.1469-7610.2007.01728.x
发表时间：
2007-06-01
影响因子：
7.6
作者：
Medina, Krista Lisdahl;Nagel, Bonnie J.;Tapert, Susan F.
通讯作者：
Tapert, Susan F.
影响因子：
3.4
作者：
Harris, LW;Sharp, T;Harrison, PJ
通讯作者：
Harrison, PJ
DOI：
10.1097/01.alc.0000179368.87886.76
发表时间：
2005-09-01
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
De Bellis, Michael D;Narasimhan, Anandhi;Clark, Duncan B
通讯作者：
Clark, Duncan B
影响因子：
--
作者：
Hommer, D;Momenan, R;Eckardt, M
通讯作者：
Eckardt, M