Primary cilia are highly oriented with respect to collagen direction and long axis of extensor tendon.
Primary cilia are highly oriented with respect to collagen direction and long axis of extensor tendon.
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DOI：
10.1002/jor.20946
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发表时间：
2010-01
影响因子：
2.8
通讯作者：
Farnum, Cornelia
中科院分区：
文献类型：
--
作者：
Donnelly, Eve;Ascenzi, Maria-Grazia;Farnum, Cornelia
骨组织通过调节基因表达、细胞代谢和细胞外基质（ECM）结构来适应机械环境；然而，这些过程的机械感觉机制尚不完全清楚。初级纤毛已经成为细胞机械感觉器官的关键组成部分，并被假设参与细胞和ECM取向的建立，但它们在骨组织中的功能才刚刚开始被研究。在这里，我们关注的是肌腱，这是一种具有定向基质的组织，非常适合分析初级纤毛和ECM之间的空间关系。本研究的目的是表征其原生ECM中小细胞初级纤毛的发生率和方向。利用多光子显微镜和半自动形态测定技术，对免疫荧光标记的大鼠伸肌腱的初级纤毛、细胞核和胶原蛋白进行了三维分析。64%的细胞中可见初级纤毛。纤毛相对于ECM高度定向：纤毛平行于胶原纤维和肌腱长轴排列。本研究首次量化了肌腱中初级纤毛的原位发生率和方向。
Skeletal tissues adapt to their mechanical environments by modulating gene expression, cell metabolism, and extracellular matrix (ECM) architecture; however, the mechanosensory mechanisms for these processes are incompletely understood. Primary cilia have emerged as critical components of the cellular mechanosensory apparatus and have been hypothesized to participate in establishment of cellular and ECM orientation, but their function in skeletal tissues is just beginning to be examined. Here we focused on tendon, a tissue with an oriented matrix that is ideal for analysis of spatial relationships between primary cilia and the ECM. The objective of this study was to characterize the incidence and orientation of tenocyte primary cilia in their native ECM. Primary cilia, nuclei, and collagen were analyzed three-dimensionally in immunofluorescently labeled rat extensor tendon using multiphoton microscopy and semi-automated morphometry. Primary cilia were observed in 64% of tenocytes. The cilia were highly oriented with respect to the ECM: cilia were aligned parallel to the collagen fibers and the long axis of the tendon. This study represents the first quantification of the in situ incidence and orientation of primary cilia in tendon.
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DOI：
10.1002/jor.20650
发表时间：
2008-10
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Leigh DR;Abreu EL;Derwin KA
通讯作者：
Derwin KA
影响因子：
2.4
作者：
Le Graverand, MPH;Ou, Y;Rattner, JB
通讯作者：
Rattner, JB
影响因子：
3
作者：
Ascenzi, Maria-Grazia;Lenox, Michelle;Farnum, Cornelia
通讯作者：
Farnum, Cornelia
影响因子：
--
作者：
MERRILEES, MJ;FLINT, MH
通讯作者：
FLINT, MH
影响因子：
3.9
作者：
Poole, CA;Jensen, CG;Wheatley, DN
通讯作者：
Wheatley, DN