Prolactin Expression in the Baboon (Papio hamadryas) Eye.
Prolactin Expression in the Baboon (Papio hamadryas) Eye.
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DOI：
10.3390/ani12172288
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发表时间：
2022-09-03
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影响因子：
--
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中科院分区：
文献类型：
--
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在灵长类动物中，催乳素（PRL）在垂体中表达；然而，也有报道称其在垂体以外的部位表达，包括在眼睛中。狒狒是一种很好的动物模型，有助于了解这种激素在眼睛中可能发挥的作用。本研究的目的是检测胎儿和成年狒狒眼组织中催乳素及其受体的表达。此外，我们还进行了催乳素基因测序和系统发育分析，以确定人类和灵长类动物催乳素基因的进化差异。我们在胎儿和成年狒狒的所有视网膜细胞谱系中检测到了催乳素和催乳素受体的表达。在角膜、晶状体、巩膜和虹膜中未检测到这些基因的表达。催乳素和催乳素受体符合进化上的基因纯化选择假说。根据我们的研究结果，催乳素在狒狒眼睛的产前和产后内稳态中起作用。我们认为这种激素在眼睛发育过程中触发自分泌和旁分泌特异性功能。
催乳素（PRL）是一种在灵长类动物垂体的催乳素细胞中表达的激素。有报道称催乳素在垂体以外的部位表达，包括眼睛；然而，其在灵长类动物发育中的眼睛里的表达是有限的。本研究的目的是确定成年和胎儿狒狒（阿拉伯狒狒）眼组织中催乳素和催乳素受体（PRLR）（mRNA和蛋白质）的表达。方法：我们通过免疫荧光分析狒狒眼组织中的催乳素和催乳素受体。通过逆转录聚合酶链反应检测催乳素和催乳素受体的mRNA，对cDNA进行克隆和测序。此外，我们还进行了系统发育分析，以确定导致催乳素和催乳素受体灵长类基因差异的进化力量。结果：我们观察到胎儿和成年狒狒所有视网膜细胞谱系中催乳素和催乳素受体（mRNA和蛋白质）的表达。催乳素和催乳素受体符合进化上的基因纯化选择假说。结论：催乳素和催乳素受体的mRNA和蛋白质在胎儿和成年狒狒的视网膜组织中表达。催乳素可能在视网膜细胞系中触发自分泌和旁分泌特异性作用。
In primates, prolactin (PRL) is expressed in the pituitary gland; however, the extra-pituitary expression has been reported, including in the eye. The baboon is an excellent animal model for understanding the possible role of this hormone in the eye. The objective of this study was to detect PRL and its receptor expression in the eye tissues of fetal and adult baboons. Moreover, we performed PRL gene sequencing and a phylogenetic analysis to identify the evolutionary divergence in human and primate genes of PRL. We detected PRL and PRLR expression in all retinal cell lineages in fetal and adult baboons. No expression of these genes was detected in the cornea, lens, sclera, and iris. PRL and PRLR fit the hypothesis of evolutionary purifying gene selection. According to our findings, PRL has a role in prenatal and post-natal homeostasis of the baboon eye. We suggest that the hormone triggers autocrine and paracrine-specific functions in eye development. Prolactin (PRL) is a hormone expressed in lactotrophs cells of the pituitary gland in primates. Extra pituitary expression of PRL has been reported, including the eye; however, expression in the developing eye of primates is limited. The aim of the study was determining the expression of PRL and PRL receptor (PRLR) (mRNAs and proteins) in adult and fetal baboon (Papio hamadryas) ocular tissues. Methods: We analyzed PRL and PRLR in baboon eyes tissues by immunofluorescence. The mRNAs of PRL and PRLR were detected by RT-PCR, cDNA was cloned, and sequenced. Furthermore, we performed a phylogenetic analysis to identify the evolutionary forces that underlie the divergence of PRL and PRLR primate genes. Results: We observed the expression of PRL and PRLR (mRNAs and proteins) in all retinal cell lineages of fetal and adult baboon. PRL and PRLR fit the hypothesis of evolutionary purifying gene selection. Conclusions: mRNA and protein of PRL and PRLR are expressed in fetal and adult baboon retinal tissue. PRL may trigger autocrine and paracrine-specific actions in retinal cell lines.
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DOI：
10.4103/jnsbm.jnsbm_303_16
发表时间：
2017-07
期刊：
Journal of natural science, biology, and medicine
影响因子：
--
作者：
Patel JB;Chauhan JB
通讯作者：
Chauhan JB
影响因子：
3
作者：
Nunez-Amaro, Carlos D.;Moreno-Vega, Aura Ileana;Clapp, Carmen
通讯作者：
Clapp, Carmen
影响因子：
3.4
作者：
Perez-Ibave, Diana Cristina;Rodriguez-Sanchez, Iram Pablo;Barrera-Saldana, Hugo Alberto
通讯作者：
Barrera-Saldana, Hugo Alberto
影响因子：
6.7
作者：
Pérez-Ibave DC;González-Alvarez R;de La Luz Martinez-Fierro M;Ruiz-Ayma G;Luna-Muñoz M;Martínez-De-Villarreal LE;De Lourdes Garza-Rodríguez M;Reséndez-Pérez D;Mohamed-Noriega J;Garza-Guajardo R;Bautista-De-Lucío VM;Mohamed-Noriega K;Barboza-Quintana O;Arámburo-De-La-Hoz C;Barrera-Saldaña HA;Rodríguez-Sánchez IP
通讯作者：
Rodríguez-Sánchez IP
影响因子：
2
作者：
Bai, D. -P.;Hu, Y. -Q.;Li, A.
通讯作者：
Li, A.