Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013-2018) from Southeast Asia.
Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013-2018) from Southeast Asia.
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DOI：
10.3390/v13091886
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发表时间：
2021-09-21
期刊：
影响因子：
--
通讯作者：
Parida S
中科院分区：
文献类型：
--
作者：
Upadhyaya S;Mahapatra M;Mioulet V;Parida S
口蹄疫是一种危害偶蹄类动物的高度传染性疾病，具有严重的经济后果。口蹄疫在东南亚（SEA）和东亚（EA）流行，具有多种血清型，对澳大利亚和其他无口蹄疫国家构成威胁。虽然接种疫苗是预防口蹄疫爆发的最重要控制措施之一，但现有疫苗可能无法提供足够的交叉保护，以对抗在这些国家传播的口蹄疫病毒（FMDV），因为新谱系和亚谱系的入侵，如在2010年韩国（一个无口蹄疫的国家）所经历的那样，当时一种新谱系的血清型O型FMDV（Mya-98）传播到该国，导致毁灭性的经济后果。在本研究中，使用三种现有（O/HKN/6/83、O/IND/R2/75和O/PanAsia-2）和一种推定（O/MYA/2009）疫苗株和完整衣壳测序，通过病毒中和试验对从SEA和EA国家选择的共计62种血清型O（2013 - 2018）病毒进行抗原性表征。衣壳蛋白序列分析表明，在该地区流行的FMDV有三种拓扑型，即Cathay型、SEA型和中东-南亚型（ME-SA）。本研究中使用的疫苗与SEA和ME-SA病毒表现出良好的匹配。然而，最近流行的国泰拓扑型病毒均未受到任何疫苗株的保护，包括现有的国泰拓扑型疫苗（O/HKN/6/83），这表明抗原性漂移，以及在该地区监测该拓扑型并在必要时开发新疫苗株的紧迫性，尽管目前该拓扑型的存在主要限于中国、香港、台湾和越南。此外，分析了这些病毒的衣壳序列，鉴定了涉及中和抗原位点1、2和5的几个衣壳氨基酸取代，其单独或一起可以支持观察到的抗原漂移。
Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals with serious economic consequences. FMD is endemic in Southeast Asia (SEA) and East Asia (EA) with the circulation of multiple serotypes, posing a threat to Australia and other FMD-free countries. Although vaccination is one of the most important control measures to prevent FMD outbreaks, the available vaccines may not be able to provide enough cross-protection against the FMD viruses (FMDVs) circulating in these countries due to the incursion of new lineages and sub-lineages as experienced in South Korea during 2010, a FMD-free country, when a new lineage of serotype O FMDV (Mya-98) spread to the country, resulting in devastating economic consequences. In this study, a total of 62 serotype O (2013–2018) viruses selected from SEA and EA countries were antigenically characterized by virus neutralization tests using three existing (O/HKN/6/83, O/IND/R2/75 and O/PanAsia-2) and one putative (O/MYA/2009) vaccine strains and full capsid sequencing. The Capsid sequence analysis revealed three topotypes, Cathay, SEA and Middle East-South Asia (ME-SA) of FMDVs circulating in the region. The vaccines used in this study showed a good match with the SEA and ME-SA viruses. However, none of the recently circulating Cathay topotype viruses were protected by any of the vaccine strains, including the existing Cathay topotype vaccine (O/HKN/6/83), indicating an antigenic drift and, also the urgency to monitor this topotype in the region and develop a new vaccine strain if necessary, although currently the presence of this topotype is mainly restricted to China, Hong Kong, Taiwan and Vietnam. Further, the capsid sequences of these viruses were analyzed that identified several capsid amino acid substitutions involving neutralizing antigenic sites 1, 2 and 5, which either individually or together could underpin the observed antigenic drift.
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