Fate map and morphogenesis of presumptive neural crest and dorsal neural tube.
Fate map and morphogenesis of presumptive neural crest and dorsal neural tube.
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DOI：
10.1016/j.ydbio.2009.03.018
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发表时间：
2009-06-15
影响因子：
2.7
通讯作者：
Bronner-Fraser, Marianne
中科院分区：
文献类型：
--
作者：
Ezin, Akouavi M.;Fraser, Scott E.;Bronner-Fraser, Marianne
关键词：
与神经嵴细胞在小鸡中随着神经褶皱升高而被诱导的经典假设相反，最近的数据表明它们在原肠胚形成过程中已经被指定。这促使我们绘制早期鸟类胚胎中神经嵴和背神经管的起源图。通过结合局部染料注射和延时成像，我们发现神经嵴和背神经管前体存在于原肠胚广阔的新月形区域中。令人惊讶的是，静态命运图与动态共焦成像一起揭示了神经板边界比预期要宽得多，并且向尾部延伸得更多。有趣的是，我们发现假定的神经嵴的位置与从原肠胚到神经胚阶段的 BMP4 表达域广泛相关。原肠胚中已经存在一定程度的尾尾模式，尽管不完整。延时成像研究表明，神经嵴和背神经管前体经历精心设计的运动，这些运动遵循时空进展，包括收敛和延伸、重新定向、细胞混合和胚胎深处的运动。通过这些重新排列和重组运动，神经嵴和背神经管前体在区域上分离，沿着胚胎轴占据可预测的轴尾位置。这种区域化逐渐发生，并且似乎在第 7 阶段在 Hensen 结头侧水平的神经细胞中完成。
In contrast to the classical assumption that neural crest cells are induced in chick as the neural folds elevate, recent data suggest that they are already specified during gastrulation. This prompted us to map the origin of the neural crest and dorsal neural tube in the early avian embryo. Using a combination of focal dye injections and time-lapse imaging, we find that neural crest and dorsal neural tube precursors are present in a broad, crescent-shaped region of the gastrula. Surprisingly, static fate maps together with dynamic confocal imaging reveal that the neural plate border is considerably broader and extends more caudally than expected. Interestingly, we find that the position of the presumptive neural crest broadly correlates with the BMP4 expression domain from gastrula to neurula stages. Some degree of rostrocaudal patterning, albeit incomplete, is already evident in the gastrula. Time-lapse imaging studies show that the neural crest and dorsal neural tube precursors undergo choreographed movements that follow a spatiotemporal progression and include convergence and extension, reorientation, cell intermixing, and motility deep within the embryo. Through these rearrangement and reorganization movements, the neural crest and dorsal neural tube precursors become regionally segregated, coming to occupy predictable rostrocaudal positions along the embryonic axis. This regionalization occurs progressively and appears to be complete in the neurula by stage 7 at levels rostral to Hensen's node.
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影响因子：
0.7
作者：
Brodland, GW
通讯作者：
Brodland, GW
影响因子：
2.7
作者：
Elul, T;Keller, R
通讯作者：
Keller, R
影响因子：
64.8
作者：
Basch, ML;Bronner-Fraser, M;García-Castro, MI
通讯作者：
García-Castro, MI
影响因子：
64.8
作者：
HENRIQUE, D;ADAM, J;ISHHOROWICZ, D
通讯作者：
ISHHOROWICZ, D
影响因子：
64.5
作者：
LIEM, KF;TREMML, G;JESSELL, TM
通讯作者：
JESSELL, TM