A microfluidic platform for the controlled synthesis of architecturally complex liquid crystalline nanoparticles.
A microfluidic platform for the controlled synthesis of architecturally complex liquid crystalline nanoparticles.
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一种用于结构复杂的液晶纳米粒子的可控合成的微流体平台。
DOI：
10.1038/s41598-023-39205-3
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发表时间：
2023-08-04
影响因子：
4.6
通讯作者：
Elani, Yuval
中科院分区：
文献类型：
--
作者：
Pilkington, Colin P.;Contini, Claudia;Barritt, Joseph D.;Simpson, Paul A.;Seddon, John M.;Elani, Yuval
软物质纳米粒子在生物技术、治疗给药和活体成像方面的应用引起了人们的极大兴趣。支持这一点的是它们的生物兼容性、选择性靶向的潜力、诱人的药代动力学特性以及下游功能化的适应性。软物质粒子固有的形态多样性可以带来增强的功能。然而，这种多样性在临床和工业环境中仍然没有被开发，并且只有最简单的粒子结构[球形脂泡和脂类/聚合物纳米颗粒(LNPs)]被常规地利用。这在一定程度上是由于缺乏适当的合成方法。为了解决这一问题，我们设计了一种可扩展的微流控流体动力聚焦(MHF)技术，用于可控、快速和连续地生产溶致液晶(LLC)纳米粒子(立方体和六体)，即具有复杂三维和二维对称内部结构的高阶脂质组件的胶体分散体。这些粒子被认为是下一代软物质纳米载体，具有独特的融合性和物理性质。至关重要的是，与其他方法不同，我们的微流控方法可以控制LLC的大小，我们继续在模型细胞膜的融合研究中利用这一特征，其中融合对颗粒直径的依赖性很明显。我们相信，我们的平台有潜力成为未来涉及非片层软纳米颗粒的研究的工具，并预计它将允许对具有多种功能的LLC颗粒进行快速原型制作，为它们最终在工业层面的广泛应用铺平道路。
Soft-matter nanoparticles are of great interest for their applications in biotechnology, therapeutic delivery, and in vivo imaging. Underpinning this is their biocompatibility, potential for selective targeting, attractive pharmacokinetic properties, and amenability to downstream functionalisation. Morphological diversity inherent to soft-matter particles can give rise to enhanced functionality. However, this diversity remains untapped in clinical and industrial settings, and only the simplest of particle architectures [spherical lipid vesicles and lipid/polymer nanoparticles (LNPs)] have been routinely exploited. This is partially due to a lack of appropriate methods for their synthesis. To address this, we have designed a scalable microfluidic hydrodynamic focusing (MHF) technology for the controllable, rapid, and continuous production of lyotropic liquid crystalline (LLC) nanoparticles (both cubosomes and hexosomes), colloidal dispersions of higher-order lipid assemblies with intricate internal structures of 3-D and 2-D symmetry. These particles have been proposed as the next generation of soft-matter nano-carriers, with unique fusogenic and physical properties. Crucially, unlike alternative approaches, our microfluidic method gives control over LLC size, a feature we go on to exploit in a fusogenic study with model cell membranes, where a dependency of fusion on particle diameter is evident. We believe our platform has the potential to serve as a tool for future studies involving non-lamellar soft nanoparticles, and anticipate it allowing for the rapid prototyping of LLC particles of diverse functionality, paving the way toward their eventual wide uptake at an industrial level.
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DOI：
10.1002/anie.201804067
发表时间：
2019-03-04
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Barriga HMG;Holme MN;Stevens MM
通讯作者：
Stevens MM
影响因子：
7.4
作者：
Friddin, Mark S.;Elani, Yuval;Ces, Oscar
通讯作者：
Ces, Oscar
DOI：
10.1073/pnas.0901240106
发表时间：
2009-06-09
影响因子：
11.1
作者：
Leforestier, Amelie;Livolant, Francoise
通讯作者：
Livolant, Francoise
影响因子：
15
作者：
Jahn, A;Vreeland, WN;Locascio, LE
通讯作者：
Locascio, LE
影响因子：
16.6
作者：
Han, Jingjing;Liu, Kai;Yan, Xuehai
通讯作者：
Yan, Xuehai