A fully automated microfluidic PCR-array system for rapid detection of multiple respiratory tract infection pathogens.
A fully automated microfluidic PCR-array system for rapid detection of multiple respiratory tract infection pathogens.
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用于快速检测多种呼吸道感染病原体的全自动微流控 PCR 阵列系统
DOI：
10.1007/s00216-021-03171-4
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发表时间：
2021-03
影响因子：
4.3
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Huang E;Wang Y;Yang N;Shu B;Zhang G;Liu D
呼吸道感染病原体的快速准确鉴定对临床诊断和治疗以及预防病原体传播具有重要意义。为了满足这一需求，开发了一种基于微流控芯片的聚合酶链式反应阵列系统OneStart。OneStart系统使用一个封装了所有所需试剂的微流控芯片，废液也被收集并存储在芯片上。这一即用系统可以完全集成地完成21种病原体的检测，样品裂解、核酸提取/纯化、实时聚合酶链式反应(Real-time PCR)顺序在同一芯片上实现。整个分析过程在1.5小时内完成，系统自动生成测试报告。该方法的最低检测下限为1.0 × 10 3拷贝·m L−1，批间变异系数为0.0 8%~0.69%，批内变异系数为0.9~2.66%。对参考样品混合物的分析结果表明，OneStart试验的特异性为100%。对批次临床样品的分析表明，OneStart方法与实时荧光聚合酶链式反应(Real-Time-PCR)方法具有一致性。OneStart系统能够提供快速、灵敏和特异的呼吸道感染病原体检测，应用OneStart系统将有助于及时的呼吸道感染临床管理和有效防止病原体传播。图形摘要OneStart是一种即用即用的系统，可以在1.5小时内在一个微芯片上完全集成地检测21种病原体。
Rapid and accurate identification of respiratory tract infection pathogens is of utmost importance for clinical diagnosis and treatment, as well as prevention of pathogen transmission. To meet this demand, a microfluidic chip-based PCR-array system, Onestart, was developed. The Onestart system uses a microfluidic chip packaged with all the reagents required, and the waste liquid is also collected and stored on the chip. This ready-to-use system can complete the detection of 21 pathogens in a fully integrated manner, with sample lysis, nucleic acid extraction/purification, and real-time PCR sequentially implemented on the same chip. The entire analysis process is completed within 1.5 h, and the system automatically generates a test report. The lower limit-of-detection (LOD) of the Onestart assay was determined to be 1.0 × 103copies·mL−1. The inter-batch variation of cycle threshold (Ct) values ranged from 0.08% to 0.69%, and the intra-batch variation ranged from 0.9% to 2.66%. Analytical results of the reference sample mix showed a 100% specificity of the Onestart assay. The analysis of batched clinical samples showed consistency of the Onestart assay with real-time PCR. With its ability to provide rapid, sensitive, and specific detection of respiratory tract infection pathogens, application of the Onestart system will facilitate timely clinical management of respiratory tract infections and effective prevention of pathogen transmission.Graphical abstractOnestart, a ready-to-use system, can detect 21 pathogens in a fully integrated manner on a microchip within 1.5 h.
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多重与单个病毒呼吸道感染：住院儿童的病毒负荷和临床疾病严重程度。
影响因子：
4.4
作者：
Martin ET;Kuypers J;Wald A;Englund JA
通讯作者：
Englund JA
DOI：
10.1164/ajrccm/152.4_pt_2.s4
发表时间：
1995-10-01
影响因子：
24.7
作者：
DENNY, FW
通讯作者：
DENNY, FW
DOI：
10.3390/microarrays4040474
发表时间：
2015-10-20
期刊：
Microarrays (Basel, Switzerland)
影响因子：
--
作者：
Mauk MG;Liu C;Song J;Bau HH
通讯作者：
Bau HH
DOI：
10.3390/s151229783
发表时间：
2015-12-01
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Luka G;Ahmadi A;Najjaran H;Alocilja E;DeRosa M;Wolthers K;Malki A;Aziz H;Althani A;Hoorfar M
通讯作者：
Hoorfar M
一种快速、低成本、基于微流控芯片的系统，用于并行识别与临床肺炎相关的多种病原体
影响因子：
4.6
作者：
Huang G;Huang Q;Xie L;Xiang G;Wang L;Xu H;Ma L;Luo X;Xin J;Zhou X;Jin X;Zhang L
通讯作者：
Zhang L