Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.
Quantitative analysis of hyperpolarized 3He ventilation changes in mice challenged with methacholine.
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DOI：
10.1002/mrm.22311
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发表时间：
2010-03
影响因子：
3.3
通讯作者：
Driehuys, Bastiaan
中科院分区：
文献类型：
--
作者：
Mistry, Nilesh N.;Thomas, Abraham;Kaushik, S. Sivaram;Johnson, G. Allan;Driehuys, Bastiaan
使用高分辨率3He MRI来描述乙酰甲胆碱（MCh）激发小鼠的局部通气变化和气道狭窄的能力，为哮喘研究提供了新的见解。然而，为了充分利用这种新技术的价值，重要的是超越图像的视觉检查，走向自动化和定量分析。为了解决这一差距，我们描述了一种后处理方法来创建通气差异图，以更好地可视化和量化MCh挑战前后的区域通气变化。我们发现，差异图揭示了气道口径的细微变化，并突出了局灶性和弥漫性区域通气的改变。通气变化包括通气不足以及通气过度的代偿区域。差异图可以通过通气变化的直方图来量化，其中标准偏差随MCh剂量线性增加（R2=0.89）。这种分析方法被证明是更敏感的比简单的基于阈值的检测总通风缺陷。适当的扩展，这种方法应该是有用的小鼠和人类受试者的研究。
The capability to use high-resolution 3He MRI to depict regional ventilation changes and airway narrowing in mice challenged with methacholine (MCh) offers the opportunity to gain new insights into the study of asthma. However, to fully exploit the value of this novel technique, it is important to move beyond visual inspection of the images towards automated and quantitative analysis. To address this gap, we describe a post-processing approach to create ventilation difference maps to better visualize and quantify regional ventilation changes before and after MCh challenge. We show that difference maps reveal subtle changes in airway caliber, and highlight both focal and diffuse regional alterations in ventilation. Ventilation changes include both hypoventilation, as well as compensatory areas of hyperventilation. The difference maps can be quantified by a histogram plot of the ventilation changes, in which the standard deviation increases linearly with MCh dose (R2=0.89). This method of analysis is shown to be more sensitive than simple threshold-based detection of gross ventilation defects. With suitable extension, this method should be useful in studies of both mice and human subjects.
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影响因子：
2.9
作者：
Thomas, Abraham C.;Potts, Erin N.;Chen, Ben T.;Slipetz, Deborah M.;Foster, W. Michael;Driehuys, Bastiaan
通讯作者：
Driehuys, Bastiaan
影响因子：
3.3
作者：
Maï, W;Badea, CT;Johnson, GA
通讯作者：
Johnson, GA
影响因子：
8
作者：
Studholme, C;Hill, DLG;Hawkes, DJ
通讯作者：
Hawkes, DJ
影响因子：
3.3
作者：
Johnson, GA;Cates, G;Swartz, J
通讯作者：
Swartz, J
影响因子：
4.4
作者：
Altes, TA;Powers, PL;Brookeman, JR
通讯作者：
Brookeman, JR