"One-Pot" Fabrication of Highly Versatile and Biocompatible Poly(vinyl alcohol)-porphyrin-based Nanotheranostics.
"One-Pot" Fabrication of Highly Versatile and Biocompatible Poly(vinyl alcohol)-porphyrin-based Nanotheranostics.
复制标题
作者：
Luo Y;Wu H;Feng C;Xiao K;Yang X;Liu Q;Lin TY;Zhang H;Walton JH;Ajena Y;Hu Y;Lam KS;Li Y
基于纳米颗粒的治疗药物已经成为纳米医学领域的一种新范式，它将多模式的成像和治疗功能整合在一个平台上。然而，这些药物的临床转译受到制造的复杂性、材料的长期毒性和不利的生物分布的严重限制。在这里，我们报告了一种非常简单和强大的方法来开发高度通用和生物相容的聚乙烯醇-卟啉纳米颗粒(PPN)。通过一锅法制备过程，包括金属离子的络合和疏水药物的包裹，单分散纳米粒子可以由非常简单的生物相容的构建块(聚乙烯醇-卟啉偶联物)自组装而成。利用这种方法，我们可以方便地生产集光学成像、正电子发射断层扫描(PET)、光动力疗法(PDT)、光热疗法(PTT)和药物输送功能于一体的多功能PPN。PPN表现出独特的构型依赖的荧光自猝灭，以及光动力学和光热性质。PPN解离后可放大近红外荧光，为低本底荧光成像提供了可能。载阿霉素(DOX)的PPN在血液循环中的半衰期是游离DOX的53倍。在单一激发波长的近红外光照射下，PPN可以被激活，在荷瘤小鼠的肿瘤部位同时释放出活性氧、热和药物，从而达到彻底根除肿瘤的目的。由于其有机成分，在治疗研究中，PPNS通过静脉给药对小鼠没有明显的细胞毒性。这种高度通用和多功能的PPN治疗纳米平台显示了将多模式成像和治疗功能整合为个性化纳米抗癌药物的巨大潜力。
Nanoparticle-based theranostic agents have emerged as a new paradigm in nanomedicine field for integration of multimodal imaging and therapeutic functions within a single platform. However, the clinical translation of these agents is severely limited by the complexity of fabrication, long-term toxicity of the materials, and unfavorable biodistributions. Here we report an extremely simple and robust approach to develop highly versatile and biocompatible theranostic poly(vinyl alcohol)-porphyrin nanoparticles (PPNs). Through a “one-pot” fabrication process, including the chelation of metal ions and encapsulation of hydrophobic drugs, monodispersenanoparticle could be formed by self-assembly of a very simple and biocompatible building block (poly(vinyl alcohol)-porphyrin conjugate). Using this approach, we could conveniently produce multifunctional PPNs that integrate optical imaging, positron emission tomography (PET), photodynamic therapy (PDT), photothermal therapy (PTT) and drug delivery functions in one formulation. PPNs exhibited unique architecture-dependent fluorescence self-quenching, as well as photodynamic- and photothermal- properties. Near-infrared fluorescence could be amplified upon PPN dissociation, providing feasibility of low-background fluorescence imaging. Doxorubicin (DOX)-loaded PPNs achieved 53 times longer half-life in blood circulation than free DOX. Upon irradiation by near infrared light at a single excitation wavelength, PPNs could be activated to release reactive oxygen species, heat and drugs simultaneously at the tumor sites in mice bearing tumor xenograft, resulting in complete eradication of tumors. Due to their organic compositions, PPNs showed no obvious cytotoxicity in mice via intravenous administration during therapeutic studies. This highly versatile and multifunctional PPN theranostic nanoplatform showed great potential for the integration of multimodal imaging and therapeutic functions towards personalized nanomedicine against cancers.
登录
查看更多内容
影响因子：
17.1
作者：
Gilbert B;Fakra SC;Xia T;Pokhrel S;Mädler L;Nel AE
通讯作者：
Nel AE
影响因子：
8
作者：
Guo Y;Zhang Z;Kim DH;Li W;Nicolai J;Procissi D;Huan Y;Han G;Omary RA;Larson AC
通讯作者：
Larson AC
影响因子：
12.4
作者：
Chen YP;Lv JW;Liu X;Zhang Y;Guo Y;Lin AH;Sun Y;Mao YP;Ma J
通讯作者：
Ma J
影响因子：
17.1
作者：
Bhirde, Ashwinkumar A.;Chikkaveeraiah, Bhaskara V.;Srivatsan, Avinash;Niu, Gang;Jin, Albert J.;Kapoor, Ankur;Wang, Zhe;Patel, Sachin;Patel, Vyomesh;Gorbach, Alexander M.;Leapman, Richard D.;Gutkind, J. Silvio;Walker, Angela R. Hight;Chen, Xiaoyuan
通讯作者：
Chen, Xiaoyuan
锰卟啉共轭金纳米壳封装阿霉素，用于潜在的磁共振成像和光触发癌症协同治疗
影响因子：
12.4
作者：
Jing L;Liang X;Li X;Lin L;Yang Y;Yue X;Dai Z
通讯作者：
Dai Z