Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells.
Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells.
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DOI：
10.1038/s41598-021-01545-3
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发表时间：
2021-11-11
影响因子：
4.6
通讯作者：
Maeda H
中科院分区：
文献类型：
--
作者：
Yamashita K;Tomokiyo A;Ono T;Ipposhi K;Alhasan MA;Tsuchiya A;Hamano S;Sugii H;Yoshida S;Itoyama T;Maeda H
白色矿物三氧化物聚集体（WMTA）是一种根管治疗材料，已知当与次氯酸钠溶液（NaOCl）接触时会呈现深棕色。本研究旨在探讨NaOCl对WMTA盘表面性质及WMTA诱导牙周膜干细胞（PDLSCs）向成骨细胞分化的影响。将混合的WMTA（ProRoot MTA）填充到模具中以形成WMTA盘。将这些圆盘浸入蒸馏水（D-WMTA）或5% NaOCl（Na-WMTA）中。研究了它们的表面结构和Ca 2+释放水平。此外，将它们与克隆的人PDLSC系（系1-17细胞）一起培养。Na-WMTA的主要晶体结构与D-WMTA相同。在D-WMTA和Na-WMTA上发现了由多角形和针状晶体组成的球状聚集体，其中包括Ca，Si，Al，C和O。然而，在Na-WMTA上也发现了许多无定形结构。这些结构由Na和Cl组成，但不包括Ca。NaOCl浸泡也降低了整个WMTA盘的Ca 2+释放水平。用D-WMTA培养的1-17系细胞形成许多矿化结节，并表现出成骨细胞相关基因的高表达水平。然而，与Na-WMTA孵育的细胞产生少量结节，并显示成骨细胞相关基因的低表达水平。这些结果表明，NaOCl通过产生无定形结构和改变其元素分布来减少WMTA中的Ca 2+释放。NaOCl也可部分消除WMTA刺激PDLSC的成骨分化的能力。
White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1–17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs.
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影响因子：
4.3
作者：
Tomokiyo A;Yoshida S;Hamano S;Hasegawa D;Sugii H;Maeda H
通讯作者：
Maeda H
影响因子：
1.3
作者：
Garna, Devy;Kaur, Manmeet;Ghuman, Mandeep
通讯作者：
Ghuman, Mandeep
影响因子：
168.9
作者：
Seo, BM;Miura, M;Shi, ST
通讯作者：
Shi, ST
影响因子：
5
作者：
Silva, R. A. B.;Borges, A. T. N.;Silva, L. A. B.
通讯作者：
Silva, L. A. B.
影响因子：
4.2
作者：
Tang, HM;Torabinejad, M;Kettering, JD
通讯作者：
Kettering, JD