Preparation and Recognition Properties of Andrographolide Molecularly Imprinted Ploymer Microspheres
Preparation and Recognition Properties of Andrographolide Molecularly Imprinted Ploymer Microspheres
复制标题
穿心莲内酯分子印迹聚合物微球的制备及识别性能
DOI：
10.4028/www.scientific.net/amr.160-162.777
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Luo, Yongming
中科院分区：
文献类型：
--
作者：
Yin, Xiaoying;Shan, Liuqing;Han, Xiulin;Luo, Yongming
以穿心莲内酯为模板分子，采用沉淀聚合法制备了分子印迹聚合物微球。用扫描电子显微镜（SEM）对合成的MIPMs的形貌进行了表征。系统研究了功能单体、交联剂、致孔剂等合成条件对分子印迹聚合物的形貌、产率和识别性能的影响。结果表明，以DVB为交联剂制备的分子印迹聚合物形态较好，但与TRIM或EDMA的结合力较低。以TRIM为交联剂制备的分子印迹聚合物粒径小，但结合亲和力高。Scatchard分析表明，在聚合物中形成了均匀的结合位点。将对穿心莲内酯具有高亲和性和高立体选择性的分子印迹聚合物应用于固相萃取柱，有望为中草药中萜类化合物的富集和检测提供一种新的方法。
Molecular imprinted polymer micrcosheres (MIPMs) were prepared through precipitation polymerization by andrographolide using as the template molecule. The morphologies of synthesized MIPMs were characterized by scanning electronmicroscope (SEM). Systematic investigations of the influences of key synthetic conditions, including functional monomers, cross-linkers and porogens, on the morphologies, yields and the recognition properties of the MIPs were conducted. The results indicated that the morphologies of MIPs with DVB as cross-linker was perfect, but their binding affinity is lower than that of MIPs with TRIM or EDMA as cross-linkers. And particle size of MIPs with TRIM as cross-linkers is small but with high binding affinity. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The application of MIPs with high affinity and excellent stereo-selectivity toward andrographolide in solid-phase extraction (SPE) column might offer a novel method for the enrichment and determination of terpenoids compounds in the traditional herbal medicine.
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