ND-FISH-positive oligonucleotide probes for detecting specific segments of rye (Secale cereale L.) chromosomes and new tandem repeats in rye
ND-FISH-positive oligonucleotide probes for detecting specific segments of rye (Secale cereale L.) chromosomes and new tandem repeats in rye
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ND-FISH 阳性寡核苷酸探针，用于检测黑麦 (Secale Greeke L.) 染色体的特定片段和黑麦中新的串联重复序列
DOI：
10.1016/j.cj.2019.10.003
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发表时间：
2020-04
期刊：
影响因子：
6.6
通讯作者：
Fu Shulan
中科院分区：
文献类型：
--
作者：
Xi Wei;Tang Shuyao;Du Haimei;Luo Jie;Tang Zongxiang;Fu Shulan
黑麦（Secale cerealeL.）已被广泛用于改良小麦（Triticum aestivumL.）个品种寡核苷酸探针结合非变性荧光原位杂交（ND-FISH）技术为黑麦染色体的鉴定提供了一种简便、有效的方法。然而，合适的ND-FISH阳性寡核苷酸探针识别黑麦染色体的特定片段缺乏。本研究中使用了五种新的ND-FISH阳性寡核苷酸探针：Oligo-5BL.46、Oligo-5A 8080、Oligo-5A8080.1、Oligo-1AL.73和Oligo-0 R3，以及两种先前报道的寡核苷酸探针Oligo-44和Oligo-45。探针Oligo-5BL.46、Oligo-5A 8080和Oligo-44分别仅在臂1 RS、5 RS和5 RL的中间区域产生信号。探针Oligo-5A8080.1与探针Oligo-45联用可同时区分1 RS、5 RS和6 RS臂的插入区。Oligo-5A 8080和Oligo-5A8080.1揭示了5S rDNA序列在5 R染色体上的分布和多态性。探针Oligo-1AL.73仅在4 R和7 R染色体上产生信号，有助于构建改良的4 RKu染色体FISH图谱和确定小麦-黑麦4 RLKu易位染色体的4 RLKu断裂点。Oligo-0 R3在14条黑麦染色体的端粒和亚端粒区产生信号。这些寡核苷酸探针还揭示了五个新的串联重复黑麦。利用本研究报道的寡核苷酸探针，当这些染色体断裂时，1 R、5 R和6 R的短臂以及4 R和7 R的长臂可以容易地被区分。
Rye (Secale cerealeL.) has been widely used to improve wheat (Triticum aestivumL.) cultivars. Oligo probes combined with non-denaturing fluorescencein situhybridization (ND-FISH) technology provide a convenient and efficient way to identify individual rye chromosomes. However, suitable ND-FISH-positive oligo probes for recognizing specific segments of rye chromosomes are lacking. Five new ND-FISH-positive oligo probes: Oligo-5BL.46, Oligo-5A8080, Oligo-5A8080.1, Oligo-1AL.73, and Oligo-0R3, combined with two previously reported oligo probes, Oligo-44 and Oligo-45, were used in this study. Probes Oligo-5BL.46, Oligo-5A8080 and Oligo-44 produced signals only in intercalary regions of arms 1RS, 5RS, and 5RL, respectively. Probe Oligo-5A8080.1 combined with probe Oligo-45 distinguished the intercalary regions of arms 1RS, 5RS, and 6RS simultaneously. Oligo-5A8080 and Oligo-5A8080.1 revealed variation in the distribution of 5S rDNA sequences and polymorphism among 5R chromosomes. Probe Oligo-1AL.73 produced signals only on chromosomes 4R and 7R and contributed to the construction of an improved FISH map of chromosome 4RKuand to the confirmation of 4RLKubreakpoints in wheat-rye 4RLKutranslocation chromosomes. Oligo-0R3 produced signals in the telomeric and subtelomeric regions of 14 rye chromosomes. These oligo probes also revealed five new tandem repeats in rye. Using the oligo probes reported in this study, the short arms of 1R, 5R, and 6R and the long arms of 4R and 7R can be easily discriminated when these chromosomes are broken.
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