Dual bioactivities of essential oil extracted from the leaves of Artemisia argyi as an antimelanogenic versus antioxidant agent and chemical composition analysis by GC/MS.
Dual bioactivities of essential oil extracted from the leaves of Artemisia argyi as an antimelanogenic versus antioxidant agent and chemical composition analysis by GC/MS.
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DOI：
10.3390/ijms131114679
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发表时间：
2012-11-12
影响因子：
5.6
通讯作者：
Chang TM
中科院分区：
文献类型：
--
作者：
Huang HC;Wang HF;Yih KH;Chang LZ;Chang TM
本研究旨在研究艾叶挥发油的抗黑素性和抗氧化性，并对其化学成分进行分析。通过蘑菇酪氨酸酶活性测定和B16F10黑色素瘤细胞模型评价挥发油对黑素合成的抑制作用。用分光光度法检测挥发油的抗氧化能力，用气相色谱-质谱仪(GC/MS)分析挥发性化学成分。结果表明，香菇挥发油显著抑制蘑菇酪氨酸酶活性(IC50=19.16 mg/mL)，下调B16F10细胞内酪氨酸酶活性，降低黑素含量，并呈剂量依赖关系。此外，挥发油对2，2-二苯基-1-苦皮酰肼(DPPH)和2，2‘-氮基-双(3-乙基苯并噻唑啉-6-磺酸)ABTS自由基有明显的清除作用，表现出明显的还原能力。挥发油的化学成分为醚(23.66%)、醇(16.72%)、倍半萜(15.21%)、酯(11.78%)、单萜(11.63%)、酮(6.09%)、芳香族化合物(5.01%)，占其化学成分的90.10%。据预测，桉树精油中除醇以外的其他成分可能与其抗氧化活性有关。结果表明，银杏叶挥发油能抑制B16F10细胞黑色素的产生，并显示出较强的抗氧化活性。因此，精油可以作为黑色素生成的抑制剂，也可以作为护肤品中的天然抗氧化剂。
The study was aimed at investigating the antimelanogenic and antioxidant properties of essential oil when extracted from the leaves of Artemisia argyi, then analyzing the chemical composition of the essential oil. The inhibitory effect of the essential oil on melanogenesis was evaluated by a mushroom tyrosinase activity assay and B16F10 melanoma cell model. The antioxidant capacity of the essential oil was assayed by spectrophotometric analysis, and the volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The results revealed that the essential oil significantly inhibits mushroom tyrosinase activity (IC50 = 19.16 mg/mL), down-regulates B16F10 intracellular tyrosinase activity and decreases the amount of melanin content in a dose-dependent pattern. Furthermore, the essential oil significantly scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) ABTS radicals, showed an apparent reduction power as compared with metal-ion chelating activities. The chemicals constituents in the essential oil are ether (23.66%), alcohols (16.72%), sesquiterpenes (15.21%), esters (11.78%), monoterpenes (11.63%), ketones (6.09%), aromatic compounds (5.01%), and account for a 90.10% analysis of its chemical composition. It is predicted that eucalyptol and the other constituents, except for alcohols, in the essential oil may contribute to its antioxidant activities. The results indicated that essential oil extracted from A. argyi leaves decreased melanin production in B16F10 cells and showed potent antioxidant activity. The essential oil can thereby be applied as an inhibitor of melanogenesis and could also act as a natural antioxidant in skin care products.
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从Vitex negundo linn叶片中提取的精油和GC-MS化学成分分析的精油的黑色素生成与抗氧化特性的抑制作用。
DOI：
10.3390/molecules17043902
发表时间：
2012-03-30
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Huang HC;Chang TY;Chang LZ;Wang HF;Yih KH;Hsieh WY;Chang TM
通讯作者：
Chang TM
影响因子：
9.3
作者：
Büyükokuroglu, ME;Gülçin, I;Küfrevioglu, Ö
通讯作者：
Küfrevioglu, Ö
影响因子：
--
作者：
Huang, Huey-Chun;Wang, Hsiao-Fen;Chang, Tsong-Min
通讯作者：
Chang, Tsong-Min
影响因子：
3.6
作者：
BALINA, LM;GRAUPE, K
通讯作者：
GRAUPE, K
影响因子：
5.4
作者：
Gülçin, I;Oktay, M;Aslan, A
通讯作者：
Aslan, A