Microbiota-accessible fiber activates short-chain fatty acid and bile acid metabolism to improve intestinal mucus barrier in broiler chickens.
Microbiota-accessible fiber activates short-chain fatty acid and bile acid metabolism to improve intestinal mucus barrier in broiler chickens.
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微生物可及纤维激活短链脂肪酸和胆汁酸代谢改善肉鸡肠道粘液屏障。
DOI：
10.1128/spectrum.02065-23
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发表时间：
2024-01-11
影响因子：
3.7
通讯作者：
Yang, Xiaojun
中科院分区：
文献类型：
--
作者：
Yang, Jiantao;Qin, Kailong;Sun, Yanpeng;Yang, Xiaojun
膳食多糖与肠道菌群和肠道功能稳态密切相关。本研究旨在探讨β-葡聚糖、阿拉伯木聚糖和抗性淀粉选择性调节肠道菌群及其代谢产物以保护肉鸡肠道粘液屏障的机制。本研究以β-葡聚糖、阿拉伯木聚糖和抗性淀粉为唯一碳源，对21日龄肉仔鸡盲肠菌群进行体外培养。我们发现β-葡聚糖、阿拉伯木聚糖和抗性淀粉分别改变了类杆菌、乳酸杆菌、粪球菌、丁酸球菌、瘤胃球菌和布劳特氏菌的群落结构并选择性地靶向富集。值得注意的是，用阿拉伯木聚糖和抗性淀粉而不是β-葡聚糖补充纤维缺乏的饮食，通过盲肠微生物群富集和微生物群衍生的短链脂肪酸（SCFA）浓度的增加来改善肠粘液屏障。此外，我们通过体外细菌培养说明，阿拉伯木聚糖和抗性淀粉的补充导致了次级胆汁酸的细菌生物转化的变化。我们的研究结果提供了关于阿拉伯木聚糖和抗性淀粉如何选择性靶向肠道微生物群并介导SCFA的产生和胆汁酸生物转化以改善肠道粘液屏障功能的见解。肠粘液屏障位于肠上皮和微生物群的界面，是抵抗病原微生物和环境抗原的第一道防线。膳食多糖作为微生物可及纤维，在肠道微生物群落的调节中起着关键作用。然而，膳食纤维通过有针对性地调节肠道微生物群来影响肠道粘液屏障的机制尚不清楚。本研究为肉鸡膳食纤维补充剂通过有针对性地调节肠道生态系统来改善肠道粘液屏障的益处提供了基本证据。我们的研究结果表明，微生物群可接近的纤维-肠道微生物群-短链脂肪酸/胆汁酸轴在调节肠道功能中起着关键作用。
Dietary polysaccharides are closely associated with gut microbiota and intestinal function homeostasis. This study aimed to investigate the mechanisms by which the β-glucan, arabinoxylan, and resistant starch selectively modulate gut microbiota and microbiota-derived metabolites to protect the intestinal mucus barrier in broiler chicken. In the present study, cecal microbiota samples from 21-day-old broilers were cultured in vitro with β-glucan, arabinoxylan, or resistant starch as the sole carbon source. We found that β-glucan, arabinoxylan and resistant starch alter community structure and selectively target the enrichment of Bacteroides, Lactobacillus, Coprococcus, Butyricicoccus, Ruminococcus and Blautia, respectively. Notably, supplementing fiber-deficient diets with arabinoxylan and resistant starch, but not with β-glucan, improved the intestinal mucus barrier by cecal microbiota enrichment and increase in the concentration of microbiota-derived short-chain fatty acids (SCFAs). In addition, we illustrated through bacterial cultures in vitro that the supplementation of arabinoxylan and resistant starch resulted in a change in bacterial biotransformation of secondary bile acids. Our findings provide insight into how arabinoxylan and resistant starch may selectively target gut microbiota and mediate the production of SCFAs and bile acid biotransformation to improve intestinal mucus barrier function. The intestinal mucus barrier, located at the interface of the intestinal epithelium and the microbiota, is the first line of defense against pathogenic microorganisms and environmental antigens. Dietary polysaccharides, which act as microbiota-accessible fiber, play a key role in the regulation of intestinal microbial communities. However, the mechanism via which dietary fiber affects the intestinal mucus barrier through targeted regulation of the gut microbiota is not clear. This study provides fundamental evidence for the benefits of dietary fiber supplementation in broiler chickens through improvement in the intestinal mucus barrier by targeted regulation of the gut ecosystem. Our findings suggest that the microbiota-accessible fiber-gut microbiota-short-chain fatty acid/bile acid axis plays a key role in regulating intestinal function.
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DOI：
10.1038/nri.2016.88
发表时间：
2016-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Johansson ME;Hansson GC
通讯作者：
Hansson GC
影响因子：
4.4
作者：
Wei Guo;Di Han;Fei Zhang;Qinyi Zhan;Yanyan Liu;Qingyun Peng;Shengshu Huang;Zhen Xue;Xiaojun Yang
通讯作者：
Xiaojun Yang
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.3
作者：
Kim, Eunjoo;Morgan, Natalie K.;Moss, Amy F.;Li, Lily;Ader, Peter;Choct, Mingan
通讯作者：
Choct, Mingan
DOI：
10.1016/j.jcmgh.2021.02.007
发表时间：
2021
影响因子：
7.2
作者：
Ghosh S;Whitley CS;Haribabu B;Jala VR
通讯作者：
Jala VR