In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
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DOI：
10.1371/journal.pone.0123980
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Miekisch W
中科院分区：
文献类型：
--
作者：
Bergmann A;Trefz P;Fischer S;Klepik K;Walter G;Steffens M;Ziller M;Schubert JK;Reinhold P;Köhler H;Miekisch W
鸟分枝杆菌亚种副结核病（MAP）是反刍动物慢性肠道疾病的病原体。可用的诊断测试复杂且缓慢。在体外，挥发性有机化合物（VOC）的模式发射MAP文化反映细菌的生长，使不同菌株的区别。本研究旨在在动物模型的受控环境中测定体内VOC。通过针阱微萃取（breath）和固相微萃取（feces）对42只山羊（16只对照组和26只接种MAP的动物）的呼吸和粪便中的VOCs进行预浓缩，并通过气相色谱/质谱（GC/MS）进行分析。在接种后18、29、33、41和48周进行分析。从血液中测定MAP特异性抗体和MAP特异性干扰素-γ-应答。通过分析纯参比物质确认所有标记物-VOC的特性。基于高pptV的检测限和两个数量级的线性范围，可以在呼吸和粪便上方的顶空中检测到超过100种VOC。28种物质在接种和未接种动物之间存在差异。尽管在感染过程中，呋喃、含氧物质和碳氢化合物等最主要物质的模式发生了变化，但接种和未接种动物之间的差异在任何时间仍可检测到粪便中的16种物质和呼吸中的3种VOC。粪便中VOC浓度的差异反映了MAP细菌的存在。呼吸中VOC谱的差异与宿主的干扰素-γ反应有关。从某种角度来看，VOCs的体内分析可能有助于克服现有测试的局限性。
Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of a chronic enteric disease of ruminants. Available diagnostic tests are complex and slow. In vitro, volatile organic compound (VOC) patterns emitted from MAP cultures mirrored bacterial growth and enabled distinction of different strains. This study was intended to determine VOCs in vivo in the controlled setting of an animal model. VOCs were pre-concentrated from breath and feces of 42 goats (16 controls and 26 MAP-inoculated animals) by means of needle trap microextraction (breath) and solid phase microextraction (feces) and analyzed by gas chromatography/ mass spectrometry. Analyses were performed 18, 29, 33, 41 and 48 weeks after inoculation. MAP-specific antibodies and MAP-specific interferon-γ-response were determined from blood. Identities of all marker-VOCs were confirmed through analysis of pure reference substances. Based on detection limits in the high pptV and linear ranges of two orders of magnitude more than 100 VOCs could be detected in breath and in headspace over feces. Twenty eight substances differed between inoculated and non-inoculated animals. Although patterns of most prominent substances such as furans, oxygenated substances and hydrocarbons changed in the course of infection, differences between inoculated and non-inoculated animals remained detectable at any time for 16 substances in feces and 3 VOCs in breath. Differences of VOC concentrations over feces reflected presence of MAP bacteria. Differences in VOC profiles from breath were linked to the host response in terms of interferon-γ-response. In a perspective in vivo analysis of VOCs may help to overcome limitations of established tests.
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肠易激综合征中粪便挥发性有机代谢产物的研究。
影响因子：
3.7
作者：
Ahmed I;Greenwood R;Costello Bde L;Ratcliffe NM;Probert CS
通讯作者：
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影响因子：
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作者：
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发表时间：
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影响因子：
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作者：
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影响因子：
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