A role for gut microbiota in early-life stress-induced widespread muscle pain in the adult rat.
A role for gut microbiota in early-life stress-induced widespread muscle pain in the adult rat.
复制标题
DOI：
10.1177/17448069211022952
复制
发表时间：
2021-01
期刊：
影响因子：
3.3
通讯作者：
Levine JD
中科院分区：
文献类型：
--
作者：
Green PG;Alvarez P;Levine JD
成年大鼠经历了新生儿有限床上（NLB），一种早期生活压力的形式，经历了持续的肌肉机械痛觉过敏。由于越来越多的人认识到肠道微生物组调节疼痛和伤害感受，并且早期生活压力对肠道微生物组产生持久的影响，我们测试了成年NLB大鼠的持续性肌肉痛觉过敏可以通过改变肠道微生物组的干预来改善的假设。成年NLB大鼠在其饮用水中给予益生菌，鼠李糖乳杆菌GG（100亿CFU/150 ml）或德西蒙制剂（DSF）（由8种细菌组成的1125亿CFU/150 ml混合物），或非吸收性抗生素利福昔明或新霉素与饼干面团混合，提供50 mg/kg。在给予益生菌或抗生素之前和之后的几个时间点评估腓肠肌的机械伤害感觉阈值。成年NLB大鼠喂食益生菌鼠李糖或DSF、抗生素，以及喂食不可吸收的抗生素利福昔明或新霉素，肌肉机械痛觉过敏明显减轻。我们假设NLB应激引起的持续性骨骼肌痛觉过敏可能至少部分是由于肠道微生物组的作用，而使用益生菌或不可吸收的抗生素调节肠道微生物组可能是治疗慢性肌肉骨骼疼痛的新方法。
Adult rats that experienced neonatal limited bedding (NLB), a form of early-life stress, experience persistent muscle mechanical hyperalgesia. Since there is a growing recognition that the gut microbiome regulates pain and nociception, and that early-life stress produces a long-lasting impact on the gut microbiome, we tested the hypothesis that persistent muscle hyperalgesia seen in adult NLB rats could be ameliorated by interventions that modify the gut microbiome. Adult NLB rats received probiotics, either Lactobacillus rhamnosus GG (10 billion CFU/150 ml) or De Simone Formulation (DSF) (112.5 billion CFU/150 ml mixture of 8 bacterial species), in their drinking water, or non-absorbable antibiotics, rifaximin or neomycin, admixed with cookie dough, to provide 50 mg/kg. Mechanical nociceptive threshold in the gastrocnemius muscle was evaluated before and at several time points after administration of probiotics or antibiotics. Adult NLB rats fed probiotics L. Rhamnosus or DSF, antibiotics, as well as rats fed non-absorbable antibiotics rifaximin or neomycin, had markedly attenuated muscle mechanical hyperalgesia. We hypothesize that persistent skeletal muscle hyperalgesia produced by NLB stress may be, at least in part, due to a contribution of the gut microbiome, and that modulation of gut microbiome using probiotics or non-absorbable antibiotics, may be novel therapeutic approaches for the treatment of chronic musculoskeletal pain.
登录
查看更多内容
DOI：
10.1016/j.ynpai.2020.100045
发表时间：
2020-01-01
期刊：
Neurobiology of pain (Cambridge, Mass.)
影响因子：
--
作者：
Dworsky-Fried, Zoe;Kerr, Bradley J;Taylor, Anna M W
通讯作者：
Taylor, Anna M W
影响因子：
4.3
作者：
Bonaz B;Bazin T;Pellissier S
通讯作者：
Pellissier S
影响因子：
3.7
作者：
Distrutti E;Cipriani S;Mencarelli A;Renga B;Fiorucci S
通讯作者：
Fiorucci S
影响因子：
4.6
作者：
Fukui H;Oshima T;Tanaka Y;Oikawa Y;Makizaki Y;Ohno H;Tomita T;Watari J;Miwa H
通讯作者：
Miwa H
影响因子：
7.3
作者：
Cristofori F;Dargenio VN;Dargenio C;Miniello VL;Barone M;Francavilla R
通讯作者：
Francavilla R