In vitro inhibition of the growth of Helicobacter hepaticus by various antibiotics
In vitro inhibition of the growth of Helicobacter hepaticus by various antibiotics
复制标题
各种抗生素对肝螺杆菌生长的体外抑制作用
DOI：
10.5897/ajb11.3808
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发表时间：
2012-03
影响因子：
--
通讯作者：
王江滨
中科院分区：
文献类型：
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作者：
王江滨
本研究考察了多种抗生素对肝螺杆菌(HH)的体外抑制和根除作用，分析了单一药物敏感抗生素联合应用对HH的抑制作用，采用E-test法和Kirby-Bauer纸片扩散法分别测定了HH标准株(ATCC51449，美式培养库)和一株分离物的最低抑菌浓度(MIC)和抑菌圈直径，并用棋盘稀释法测定其对左氧氟沙星、莫西沙星和美罗培南高度敏感；对阿莫西林、氧氟沙星、克拉霉素、替硝唑、甲硝唑、四环素敏感；对头孢菌素、糖肽类抗生素不敏感。阿莫西林与甲硝唑、克拉霉素、左氧氟沙星、四环素合用(FIC指数为0.5)显示协同抑制作用，克拉霉素与哌拉西林他唑巴坦、甲硝唑美罗培南、甲硝唑哌拉西林他唑巴坦、美罗培南合用呈累积效应(FIC指数为0.5~1)。阿莫西林与哌拉西林-他唑巴坦联用、四环素与哌拉西林-他唑巴坦联用显示出独立作用(FIC指数从1到2)。这些发现有望为进一步筛选对HHH敏感的菌株，为未来临床根除HH菌株，避免最大限度的耐药提供实验依据。
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关键词：肝螺杆菌，抑制作用，敏感性，抗生素。
The present study investigated the in vitro inhibiting and eradicating effect ofHelicobacter hepaticus (Hh) by various antibiotics, and analyzed the inhibiting effect of the combined application of single-drug sensitive antibiotics to Hh. E-test method and Kirby-Bauer disk agar diffusion method were used to detect the minimum inhibitory concentration (MIC) and inhibition zone diameter of a standard Hh strain (ATCC51449, American Type Culture Collection) and an isolate, respectively. Then the checkerboard dilution method was used to determine the fractional inhibitory concentration (FIC) and the combined sensitivity effect. Hh standard strain ATCC51449 and the isolate were highly sensitive to levofloxacin, moxifloxacin and meropenem; sensitive to amoxicillin, ofloxacin, clarithromycin, tinidazole, metronidazole and tetracycline; whereas not sensitive to the cephalosporins and glycopeptide antibiotics. Amoxicillin combined with metronidazole, clarithromycin, levofloxacin, or tetracycline (FIC index <0.5) showed synergistic inhibitory effect; clarithromycin combined with piperacillin tazobactam, metronidazole meropenem, metronidazole piperacillin tazobactam and meropenem combined with piperacillin tazobactam showed cumulative effect (FIC index from 0.5 to 1). In contrast, amoxicillin combined with piperacillin-tazobactam, as well as tetracycline combined with piperacillin tazobactam showed an independant effect (FIC index from 1 to 2). These findings are expected to provide an experimental basis for further screening of the antibioticssensitive to Hh for future clinical eradication of Hh and avoiding resistance at maximum limit.
Key words: Helicobacter hepaticus, inhibitory effect, sensitivity, antibiotics.
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DOI：
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发表时间：
1995-08
期刊：
Laboratory animal science
影响因子：
--
作者：
R. Russell;D. Haines;M. Anver;J. Battles;P. Gorelick;L. L. Blumenauer-L.;Gonda;Jerrold M. Ward
通讯作者：
R. Russell;D. Haines;M. Anver;J. Battles;P. Gorelick;L. L. Blumenauer-L.;Gonda;Jerrold M. Ward
影响因子：
11.2
作者：
Rao, Varada P.;Poutahidis, Theofilos;Erdman, Susan E.
通讯作者：
Erdman, Susan E.
影响因子：
4.9
作者：
E. Lin;R. Stanek;M. Mufson
通讯作者：
E. Lin;R. Stanek;M. Mufson
DOI：
10.1128/cdli.9.6.1160-1164.2002
发表时间：
2002-11-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Ananieva, O;Nilsson, I;Wadström, T
通讯作者：
Wadström, T
影响因子：
3.1
作者：
Cahill, RJ;Foltz, CJ;Schauer, DB
通讯作者：
Schauer, DB