The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus.
The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus.
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DOI：
10.1186/s12870-021-03336-4
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发表时间：
2021-12-29
影响因子：
5.3
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Wang D;Sun Z;Hu X;Xiong J;Hu L;Xu Y;Tang Y;Wu Y
APETALA 2/乙烯反应因子（AP 2/ERF）家族是植物对环境刺激反应的重要调控因子。然而，它们在百脉根耐盐性中的作用尚不清楚。在这里，关键的盐响应转录因子LcERF 056被克隆和表征。属于ERF的B3-1（IX）亚家族的LcERF 056被盐处理显著上调。LcERF 056融合的GFP仅定位于细胞核。此外，LcERF 056过表达（OE）转基因拟南芥和L.与野生型（WT）或RNA干扰表达（RNAi）转基因株系相比，小角藻株系在表型和生理水平上表现出显著高的耐盐性。OE、RNAi和WT系的转录组分析表明，LcERF 056调节参与多种代谢途径的下游基因。染色质免疫沉淀-定量聚合酶链反应（ChIP-qPCR）和酵母单杂交（Y1 H）实验表明，LcERF 056可与脂转运蛋白、过氧化物酶和核糖体蛋白等活性氧相关基因的GCC盒或DRE顺式元件结合。结果表明，关键调节子LcERF 056在苜蓿耐盐性中起重要作用。通过调节ROS相关基因来调控小角海鞘的生长。因此，它可能是一个有用的目标，耐盐L。玉米或其他作物。在线版本包含补充材料，可通过10.1186/s12870-021-03336-4获得。
The APETALA2/ethylene response factor (AP2/ERF) family are important regulatory factors involved in plants’ response to environmental stimuli. However, their roles in salt tolerance in Lotus corniculatus remain unclear. Here, the key salt-responsive transcription factor LcERF056 was cloned and characterised. LcERF056 belonging to the B3–1 (IX) subfamily of ERFs was considerably upregulated by salt treatment. LcERF056-fused GFP was exclusively localised to nuclei. Furthermore, LcERF056- overexpression (OE) transgenic Arabidopsis and L. corniculatus lines exhibited significantly high tolerance to salt treatment compared with wild-type (WT) or RNA interference expression (RNAi) transgenic lines at the phenotypic and physiological levels. Transcriptome analysis of OE, RNAi, and WT lines showed that LcERF056 regulated the downstream genes involved in several metabolic pathways. Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) and yeast one-hybrid (Y1H) assay demonstrated that LcERF056 could bind to cis-element GCC box or DRE of reactive oxygen species (ROS)-related genes such as lipid-transfer protein, peroxidase and ribosomal protein. Our results suggested that the key regulator LcERF056 plays important roles in salt tolerance in L. corniculatus by modulating ROS-related genes. Therefore, it may be a useful target for engineering salt-tolerant L. corniculatus or other crops. The online version contains supplementary material available at 10.1186/s12870-021-03336-4.
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