Pathophysiological analyses of skeletal muscle in obese type 2 diabetes SDT fatty rats.
Pathophysiological analyses of skeletal muscle in obese type 2 diabetes SDT fatty rats.
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DOI：
10.1293/tox.2017-0064
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发表时间：
2018-04
影响因子：
1.2
通讯作者：
Yamada T
中科院分区：
文献类型：
--
作者：
Kemmochi Y;Ohta T;Motohashi Y;Kaneshige A;Katsumi S;Kakimoto K;Yasui Y;Anagawa-Nakamura A;Toyoda K;Taniai-Riya E;Takahashi A;Shoda T;Yamada T
肌肉减少症是与年龄相关的肌肉质量和功能下降。糖尿病和肥胖是导致肌肉减少症的危险因素，但糖尿病相关肌肉减少症的潜在机制尚不清楚。肥胖型2型糖尿病SDT肥胖大鼠表现为早期严重糖尿病，其骨骼肌的特点尚未见报道。因此，对这些大鼠的骨骼肌进行病理生理学分析。在8、16、24、32和40周龄处死糖尿病雄性SDT脂肪大鼠。正常对照组为SD（Sprague道利）大鼠。除了生物血液参数，比目鱼肌和趾长伸肌肌肉的肌肉重量，组织病理学，蛋白质的合成和降解进行了检查。还检查了肌肉握力。这些结果表明，SDT脂肪大鼠的肌肉重量从16周龄开始显著降低。SDT脂肪大鼠的肌纤维平均横截面积从24周龄开始下降。从8周龄开始，SDT脂肪大鼠中观察到肌细胞内脂质积聚增加，通过嗜脂蛋白免疫组织化学和TEM鉴定。SDT肥胖大鼠的血浆胰岛素样生长因子（IGF）-1水平和肌肉力量在24周龄及以后下降。这些病理生理学发现在老年人和糖尿病患者的肌肉减少症中都有报道。总之，SDT肥胖大鼠被认为是一个有用的模型，用于分析糖尿病相关的肌肉减少症。
Sarcopenia is the age-related decrease of muscle mass and function. Diabetes and obesity are known to be risk factors that exacerbate sarcopenia, but the underlying mechanism of diabetes-related sarcopenia is still unknown. Obese type 2 diabetes SDT fatty rats show early onset of severe diabetes and there have been no reports on the characteristics of their skeletal muscle. Therefore, pathophysiological analyses were performed for the skeletal muscle in these rats. Diabetic male SDT fatty rats were sacrificed at 8, 16, 24, 32 and 40 weeks of age. Age-matched Sprague Dawley (SD) rats were used as the normal control. In addition to biological blood parameters, the soleus and the extensor digitorum longus muscles were examined for muscle weight, histopathology, and protein synthesis and degradation. Muscle grip strength was also examined. These results revealed that the muscle weights of the SDT fatty rats were significantly decreased from 16 weeks of age. The mean cross-sectional area of muscle fibers in the SDT fatty rats decreased from 24 weeks of age. Increased intramyocellular lipid accumulation, identified by immunohistochemistry for adipophilin and TEM, was observed in the SDT fatty rats from 8 weeks of age. Plasma insulin-like growth factor (IGF)-1 levels and muscle strength in the SDT fatty rats decreased at 24 weeks of age and thereafter. These pathophysiological findings have been reported both in sarcopenia in aged humans and in patients with diabetes. In conclusion, the SDT fatty rat was considered to be a useful model for analysis of diabetes-related sarcopenia.
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影响因子：
2.4
作者：
Matsui, Kenichi;Ohta, Takeshi;Matsushita, Mutsuyoshi
通讯作者：
Matsushita, Mutsuyoshi
影响因子：
2.2
作者：
Morley, John E.
通讯作者：
Morley, John E.
影响因子：
5.8
作者：
Benbassat, CA;Maki, KC;Unterman, TG
通讯作者：
Unterman, TG
与年龄和疾病相关的肌肉损失：糖尿病、肥胖和其他疾病的影响。
影响因子：
44.5
作者：
Kalyani, Rita Rastogi;Corriere, Mark;Ferrucci, Luigi
通讯作者：
Ferrucci, Luigi
DOI：
10.1046/j.1365-2281.2000.00269.x
发表时间：
2000-09-01
期刊：
CLINICAL PHYSIOLOGY
影响因子：
--
作者：
Cederholm, T;Sylvén, C;Jansson, E
通讯作者：
Jansson, E