Investigating the effects of conductivity on zone overlap with EMMA: computer simulation and experiment.
Investigating the effects of conductivity on zone overlap with EMMA: computer simulation and experiment.
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DOI：
10.1002/elps.201000451
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发表时间：
2011-06
期刊：
影响因子：
2.9
通讯作者：
Strein, Timothy G.
中科院分区：
文献类型：
--
作者：
Stahl, John W.;Catherman, Adam D.;Sampath, Ranasinghe K.;Seneviratne, C. Aravinda;Strein, Timothy G.
在本文中，我们演示，使用实验和模拟，如何样品区的电导率可以影响塞塞混合在小分子的应用中的电介质介导的微量分析（EMMA）。在线混合的有效性，这是由潜在的驱动，可以有很大的变化与实验条件。使用两个小分子系统，分析物插头，试剂插头和背景电解质之间的局部电导率差异的EMMA分析的影响进行了检查。Simul 5.0是一个动态模拟程序，用于毛细管电泳（CE）系统，用于了解离子边界和配置文件，产生实验获得的数据EMMA分析（i）肌酐测定通过Jaffe反应，涉及中性和阴离子的反应，和（ii）没食子酸和二氯靛酚（DCIP），两个阴离子之间的氧化还原反应。在CE分析中广泛使用的低样品电导率对于涉及中性反应物的在线反应可能是有害的，因为离子组分穿过低电导率中性区的快速迁移导致不良的试剂塞重叠和低反应效率。相反，对于两种类似带电的试剂，低电导率样品塞是有利的，因为它允许试剂场放大堆叠到紧密的反应区中。此外，同时重叠的三个试剂区的复杂性被认为是，实验结果验证了模拟所作的预测。然而，模拟似乎并不能预测所有观察到的实验行为。总体而言，通过实验与模拟相结合，实现了对EMMA中局部场效应的增强认识，并且可以为EMMA分析的类别建立有利的样本矩阵的一般准则。
In this paper we demonstrate, using both experiment and simulation, how sample zone conductivity can affect plug-plug mixing in small molecule applications of electrophoretically mediated microanalysis (EMMA). The effectiveness of in-line mixing, which is driven by potential, can vary widely with experimental conditions. Using two small molecule systems, the effects of local conductivity differences between analyte plugs, reagent plugs and the background electrolyte on EMMA analyses are examined. Simul 5.0, a dynamic simulation program for capillary electrophoresis (CE) systems, is used to understand the ionic boundaries and profiles that give rise to the experimentally obtained data for EMMA analyses for (i) creatinine determination via the Jaffe reaction, a reaction involving a neutral and an anion, and (ii) the redox reaction between gallate and dichloroindophenol (DCIP), two anions. Low sample conductivity, which is widely used in CE analyses, can be detrimental for in-line reactions involving a neutral reactant, as rapid migration of the ionic component across a low conductivity neutral zone results in poor reagent plug overlap, and low reaction efficiency. Conversely, with two similarly charged reagents, a low conductivity sample plug is advantageous, as it allows field-amplified stacking of the reagents into a tight reaction zone. In addition, the complexity of simultaneously overlapping three reagent zones is considered, and experimental results validate the predictions made by the simulation. The simulations, however, do not appear to predict all of the observed experimental behavior. Overall, by combining experiment with simulation, an enhanced appreciation for the local field effects in EMMA is realized, and general guidelines for an advantageous sample matrix can be established for categories of EMMA analyses.
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影响因子：
6.1
作者：
Merola, Emily T.;Catherman, Adam D.;Strein, Timothy G.
通讯作者：
Strein, Timothy G.
影响因子：
3.6
作者：
AVILA, LZ;WHITESIDES, GM
通讯作者：
WHITESIDES, GM
影响因子：
7.4
作者：
HARMON, BJ;PATTERSON, DH;REGNIER, FE
通讯作者：
REGNIER, FE
影响因子：
7.4
作者：
Sanders, BD;Slotcavage, RL;Strein, TG
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Strein, TG
影响因子：
2.9
作者：
Hruska, V;Jaros, M;Gas, B
通讯作者：
Gas, B