Serologic and Virologic Survey for Evidence of Infection with Velogenic Newcastle Disease Virus in Chinese Duck Farms
Serologic and Virologic Survey for Evidence of Infection with Velogenic Newcastle Disease Virus in Chinese Duck Farms
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中国养鸭场强效新城疫病毒感染证据的血清学和病毒学调查
DOI：
10.1637/9649-010611-resnote.1
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发表时间：
2011-09
期刊：
影响因子：
1.4
通讯作者：
Zhang Guozhong
中科院分区：
文献类型：
--
作者：
Zhang Shouping;Zhao Lili;Wang Xiaoting;Zhang Dabing;Zhao Jixun;Zhang Guozhong
摘要为了解我国鸭群中纽卡斯尔病毒（NDV）的感染情况，进行了血清学和病毒学调查。在调查所涵盖的两个地理区域的12个抽样农场中，有9个检测到NDV感染。406份血清标本中抗体阳性率为35.7%。通过血凝试验、血凝抑制试验、致病性试验以及融合基因的逆转录-聚合酶链反应（RT-PCR），从不同鸭群中分离到3株新城疫病毒（NDV）。系统发育分析表明，所有三个分离物聚类与II类病毒，两个是遗传学接近基因型VII NDV，和其他更密切相关的基因型IX NDV。这些结果表明，NDV感染是普遍存在的，至少有两个不同的毒力基因型可能是负责最近在中国鸭群的流行。
SUMMARY. A serologic and virologic survey was performed to determine the prevalence and distribution of Newcastle disease virus (NDV) infection in Chinese duck flocks. NDV infection was detected in nine of the 12 sampled farms throughout the two geographic regions covered by the survey. The percentage antibody positivity among the 406 serum samples was 35.7%. Three velogenic NDVs were obtained from different duck flocks identified by hemagglutination and hemagglutination-inhibition tests, a pathogenicity test, and reverse transcription–polymerase chain reaction on the fusion (F) genes. Phylogenetic analysis revealed that all three isolates clustered with the class II viruses; two were phylogenetically close to genotype VII NDVs, and the other was more closely related to genotype IX NDVs. These findings suggest that NDV infections were prevalent, and at least two distinct virulent genotypes may be responsible for recent epidemics in Chinese duck flocks.
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DOI：
10.1007/978-1-4613-1759-3_2
发表时间：
1988
期刊：
--
影响因子：
--
作者：
D. Alexander
通讯作者：
D. Alexander
影响因子：
3.3
作者：
Hu, Beixia;Huang, Yanyan;Zhang, Xiumei
通讯作者：
Zhang, Xiumei
影响因子：
5.4
作者：
Kim, L. Mia;King, Daniel J.;Afonso, Claudio L.
通讯作者：
Afonso, Claudio L.
影响因子：
3.3
作者：
Tsai, HJ;Chang, KH;Alexander, DJ
通讯作者：
Alexander, DJ
中国东部活禽市场家鸭（绿头鸭和凯里纳）无毒力新城疫病毒监测及分离病毒特征
影响因子：
2.8
作者：
Liu, Xiaowen;Wang, Xiaoquan;Liu, Xiufan
通讯作者：
Liu, Xiufan