Functional neuronal circuitry and oscillatory dynamics in human brain organoids.
Functional neuronal circuitry and oscillatory dynamics in human brain organoids.
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DOI：
10.1038/s41467-022-32115-4
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发表时间：
2022-07-29
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
Human brain organoids replicate much of the cellular diversity and developmental anatomy of the human brain. However, the physiology of neuronal circuits within organoids remains under-explored. With high-density CMOS microelectrode arrays and shank electrodes, we captured spontaneous extracellular activity from brain organoids derived from human induced pluripotent stem cells. We inferred functional connectivity from spike timing, revealing a large number of weak connections within a skeleton of significantly fewer strong connections. A benzodiazepine increased the uniformity of firing patterns and decreased the relative fraction of weakly connected edges. Our analysis of the local field potential demonstrate that brain organoids contain neuronal assemblies of sufficient size and functional connectivity to co-activate and generate field potentials from their collective transmembrane currents that phase-lock to spiking activity. These results point to the potential of brain organoids for the study of neuropsychiatric diseases, drug action, and the effects of external stimuli upon neuronal networks. Brain organoids replicate cellular organization found in the developing human brain. Here, the authors utilize microelectronics to map activity in brain organoids and assemble functional circuits that mirror complexity found in brain networks in vivo.
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细胞外田地和电流的起源-EEG，ECOG，LFP和尖峰。
DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Buzsáki G;Anastassiou CA;Koch C
通讯作者：
Koch C
影响因子：
16.2
作者：
Buzsáki G;Logothetis N;Singer W
通讯作者：
Singer W
影响因子：
6.1
作者：
Berdondini, Luca;Imfeld, Kilian;Martinoia, Sergio
通讯作者：
Martinoia, Sergio
影响因子：
16.2
作者：
Amilhon, Benedicte;Huh, Carey Y. L.;Williams, Sylvain
通讯作者：
Williams, Sylvain
影响因子：
2.4
作者：
Bounova, Gergana;de Weck, Olivier
通讯作者：
de Weck, Olivier