The effect of modulators of radiation-induced G2 arrest on the repair of radiation-induced DNA damage detectable by neutral filter elution.
The effect of modulators of radiation-induced G2 arrest on the repair of radiation-induced DNA damage detectable by neutral filter elution.
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辐射诱导的 G2 阻滞调节剂对中性过滤洗脱可检测的辐射诱导的 DNA 损伤修复的影响。
DOI：
10.1080/09553008814552191
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发表时间：
1988
影响因子：
2.6
通讯作者：
Kort,L
中科院分区：
文献类型：
--
作者：
Rowley,R;Kort,L
有人认为，辐射诱导的g2阻滞是间期的延长，允许有丝分裂前DNA双链断裂（dsb）的修复。环己亚胺阻断损伤的修复，从而导致辐射诱导的G2arrest。咖啡因和虫草素可缩短G2arrest的持续时间。因此，所有这些药物都会降低细胞修复dsb的能力。测定了环己亚胺（50µg/ml）、咖啡因（5 mm）和虫草素（0.15 mm）对中性过滤洗脱法检测到的DNA损伤修复的影响。以20、60和100 Gy剂量的x射线照射中国仓鼠卵巢细胞（CHO），然后在没有药物治疗或每种药物存在的情况下进行长达6小时的修复。DNA损伤修复分两个阶段进行。快速组件的维修过程（约1 / 2）。7 min)未被药物治疗改变；慢速组件（约1 / 2）。170分钟)不受环己亚胺或虫草素的影响，但似乎受到咖啡因的抑制。得出的结论是：(a)导致辐射诱导的G2arrest的病变不是通过中性过滤器洗脱检测到的病变，(b)咖啡因对dsb修复的影响是咖啡因特有的，而不是通过减少G2arrest的持续时间来介导的。
It has been suggested that radiation-induced G2-arrest is an extension of interphase to allow repair of DNA double-strand breaks (dsb) prior to mitosis. Cycloheximide blocks the repair of the lesions which result in radiation-induced G2arrest. Caffeine and cordycepin reduce the duration of G2arrest. All of these agents should therefore reduce the cell's ability to repair dsb. The influence of cycloheximide (50 µg/ml), caffeine (5 mm) and cordycepin (0·15 mm) on the repair of the damage detectable in DNA by neutral filter elution was determined. Chinese hamster ovary cells (CHO) were irradiated with X-ray doses of 20, 60 and 100 Gy then allowed to repair without drug treatment or in the presence of each drug for intervals up to 6 h. DNA damage repair proceeded in two phases. The fast component of the repair process (t½approx. 7 min) was not modified by drug treatment; the slow component (t½approx. 170 min) was unaffected by cycloheximide or cordycepin, but appeared to be inhibited by caffeine. It was concluded that: (a) the lesion which results in radiation-induced G2arrest is not the lesion which is detectable by neutral filter elution, and (b) the influence of caffeine on dsb repair is specific to caffeine and is not mediated by a reduction in the duration of G2arrest.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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发表时间：
1985
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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