Fish oil and inflammatory status alter the n-3 to n-6 balance of the endocannabinoid and oxylipin metabolomes in mouse plasma and tissues.
Fish oil and inflammatory status alter the n-3 to n-6 balance of the endocannabinoid and oxylipin metabolomes in mouse plasma and tissues.
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DOI：
10.1007/s11306-012-0421-9
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发表时间：
2012-12
期刊：
影响因子：
3.6
通讯作者：
Witkamp, Renger F.
中科院分区：
文献类型：
--
作者：
Balvers, Michiel G. J.;Verhoeckx, Kitty C. M.;Bijlsma, Sabina;Rubingh, Carina M.;Meijerink, Jocelijn;Wortelboer, Heleen M.;Witkamp, Renger F.
已经确定的是，n-3脂肪酸的饮食摄入与抗炎作用相关，并且这与氧脂素和内源性大麻素代谢组的调节有关。然而，关于特定组织效应的数据量是有限的，并且不知道炎症如何影响这种关系。在本研究中，我们系统地探讨了n-3脂肪酸饮食和炎症对体内内源性大麻素和氧脂代谢组的综合影响，使用多室，详细的靶向脂质组学方法。雄性C57 BL/6小鼠接受含有0、1或3%w/w鱼油（FO）的饮食6周，之后腹膜内施用2 mg/kg LPS或盐水。FO通常增加了“n-3”NAE和氧脂素，而消耗了衍生自其它脂肪酸的化合物，影响氧脂素代谢组的所有分支。LPS通常增加内源性大麻素/NAE和氧化脂质的水平，在血浆和组织中具有相反的作用。多变量数据分析显示，盐水处理组中饮食组之间的分离主要由n-3衍生化合物以外的化合物减少解释。在LPS处理组中，分离主要通过n-3衍生化合物的增加来解释。总之，FO引起内源性大麻素和氧脂素代谢组的n-3至n-6平衡的显著变化，具体影响取决于炎症状态。本文的在线版本（doi：10.1007/s11306-012-0421-9）包含补充材料，可供授权用户使用。
It is well established that dietary intake of n-3 fatty acids is associated with anti-inflammatory effects, and this has been linked to modulation of the oxylipin and endocannabinoid metabolomes. However, the amount of data on specific tissue effects is limited, and it is not known how inflammation affects this relation. In the present study we systematically explored the combined effects of n-3 fatty acid diets and inflammation on the in vivo endocannabinoid and oxylipin metabolomes using a multicompartment, detailed targeted lipidomics approach. Male C57BL/6 mice received diets containing 0, 1, or 3 % w/w fish oil (FO) for 6 weeks, after which 2 mg/kg LPS or saline was administered i.p. Levels of endocannabinoids/N-acylethanolamines (NAEs) and oxylipins, covering n-3 and n-6 fatty acid derived compounds, were determined in plasma, liver, ileum and adipose tissue using LC–MS/MS. FO generally increased ‘n-3’ NAEs and oxylipins at the expense of compounds derived from other fatty acids, affecting all branches of the oxylipin metabolome. LPS generally increased levels of endocannabinoids/NAEs and oxylipins, with opposing effects across plasma and tissues. Multivariate data analysis revealed that separation between diet groups in the saline treated groups was primarily explained by decreases in other than n-3 derived compounds. In the LPS treated groups, the separation was primarily explained by increases in n-3 derived compounds. In conclusion, FO caused marked changes in the n-3 to n-6 balance of the endocannabinoid and oxylipin metabolomes, with specific effects depending on inflammatory status. The online version of this article (doi:10.1007/s11306-012-0421-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.101119098
发表时间：
2001-05-22
影响因子：
11.1
作者：
Berger, A;Crozier, G;Di Marzo, V
通讯作者：
Di Marzo, V
影响因子：
7.1
作者：
Calder, Philip C.
通讯作者：
Calder, Philip C.
影响因子：
7.1
作者：
Carpentier, Yvon A.;Portois, Laurence;Malaisse, Willy J.
通讯作者：
Malaisse, Willy J.
影响因子：
3.2
作者：
Hansen, H. S.;Artmann, A.
通讯作者：
Artmann, A.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y