Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans.
Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans.
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DOI：
10.1172/jci.insight.164781
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发表时间：
2023-03-08
期刊：
影响因子：
8
通讯作者：
Yoder, Mervin C. .
中科院分区：
文献类型：
--
作者：
Lin, Yang;Banno, Kimihiko;Gil, Chang-Hyun;Myslinski, Jered;Hato, Takashi;Shelley, William C. .;Gao, Hongyu;Xuei, Xiaoling;Liu, Yunlong;Basile, David P. .;Yoshimoto, Momoko;Prasain, Nutan;Tarnawsky, Stefan P.;Adams, Ralf H.;Naruse, Katsuhiko;Yoshida, Junko;Murphy, Michael P. .;Horie, Kyoji;Yoder, Mervin C. .
大多数循环内皮细胞是凋亡的，但罕见的循环内皮集落形成细胞（C-ECFCs），也称为血外生内皮细胞，具有增殖和血管生成活性，可以培养；然而，这些c - ecfc的起源和原始功能仍然不清楚。在此，详细的谱系追踪显示，小鼠c - ecfc在出生后早期出现，与组织驻留的ecfc相比，显示出高的血管生成潜力，克隆增殖细胞的频率增加，并且不是致力于或来源于BM造血系统，而是来自组织驻留的ecfc。在人类中，C- ecfc存在于CD34bright脐带血单核亚群中，在幼稚或培养状态下具有增殖潜力和体内血管生成功能，并表现出单细胞转录组共享脐静脉内皮细胞的一些特征，如更高的蛋白C受体和细胞外基质基因表达。本研究通过确定起源、初始功能和抗原，为前瞻性分离c - ecfc进行转译研究提供了一项新进展。
Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine C-ECFCs emerged in the early postnatal period, displayed high vasculogenic potential with enriched frequency of clonal proliferative cells compared with tissue-resident ECFCs, and were not committed to or derived from the BM hematopoietic system but from tissue-resident ECFCs. In humans, C-ECFCs were present in the CD34bright cord blood mononuclear subset, possessed proliferative potential and in vivo vasculogenic function in a naive or cultured state, and displayed a single cell transcriptome sharing some umbilical venous endothelial cell features, such as a higher protein C receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies.
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DOI：
10.1084/jem.20072168
发表时间：
2008-05-12
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Benz C;Martins VC;Radtke F;Bleul CC
通讯作者：
Bleul CC
影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
DOI：
10.1165/rcmb.2014-0185oc
发表时间：
2015-10-01
影响因子：
6.4
作者：
Kawasaki, Takeshi;Nishiwaki, Tetsu;Tatsumi, Koichiro
通讯作者：
Tatsumi, Koichiro
影响因子：
--
作者：
Estes, Myka L;Mund, Julie A;Case, Jamie
通讯作者：
Case, Jamie
DOI：
10.1136/fn.74.2.f132
发表时间：
1996-03-01
影响因子：
4.4
作者：
Bird, JA;Spencer, JAD;Symonds, ME
通讯作者：
Symonds, ME