Development and identification of a sex-specific molecular marker in Dai-qu stock large yellow croaker (Larimichthys crocea)
Development and identification of a sex-specific molecular marker in Dai-qu stock large yellow croaker (Larimichthys crocea)
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岱曲种群大黄鱼性别特异性分子标记的建立与鉴定
DOI：
10.1016/j.aquaculture.2022.738172
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发表时间：
2022-03
期刊：
影响因子：
4.5
通讯作者：
Lou Bao
中科院分区：
文献类型：
--
作者：
Yu Min;Xie Qing-Ping;Wei Fu-Liang;Wu Xiong-Fei;Xu Wan-Tu;Zhan Wei;Liu Feng;Guo Dan-Dan;Niu Bao-Long;Lou Bao
Dai-qu stock large yellow croaker (Larimichthys crocea) is an important marine economic fish and geographical population in the East China Sea. Traditionally, large yellow croakers can be divided into three geographical populations as follows: Dai-qu, Min-yuedong and Nao-zhou stocks. In the present study, a sex-specific molecular marker in Dai-qu stock was developed from loci betweendmrt1andcfap157. Based on large yellow croaker genome sequence and a well characterized sex-determining region on chromosome 22, 147 pairs of primers were designed for screening the sex-specific markers by PCR amplification. At last, 1 pair of primers were successfully screened and identified. The results showed that the primers only amplified 500 bp bands in females, whereas, 500 bp bands and 390 bp specific bands were amplified in males. Cloning and sequencing analysis of PCR products showed that females and males share the almost same 500 bp sequence, and the males have a specific deletion of 110 bp than females sequence. 276 individuals of Dai-qu stock large yellow croaker were used to verification this sex-specific marker, and compared with gonadal histological results, the accuracy of the specific primer reached 100%, indicating that the primer can effectively and accurately identify the genetic sex of Dai-qu stock large yellow croaker. In this study, a sex-specific marker was developed and identified, which further verified that the Dai-qu stock large yellow croaker also belonged to XX♀-XY♂ type, providing a useful tool for clarifying the molecular mechanism of sex determination and promoting sex-controlled breeding.
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DOI：
10.1093/jhered/esi019
发表时间：
2005-03
期刊：
The Journal of heredity
影响因子：
--
作者：
G. Perry;M. Ferguson;Takashi Sakamoto;R. Danzmann
通讯作者：
G. Perry;M. Ferguson;Takashi Sakamoto;R. Danzmann
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
Shen Xi
通讯作者：
Shen Xi
DOI：
10.1002/9781119127291.ch39
发表时间：
2018-11
期刊：
Sex Control in Aquaculture
影响因子：
--
作者：
Zhi-Yong Wang;Mingyi Cai
通讯作者：
Zhi-Yong Wang;Mingyi Cai
尼罗罗非鱼 (Oreochromis niloticus) 性连锁 DNA 标记的筛选和表征以及标记辅助选择
影响因子：
4.5
作者：
Yang C;Yang SJ;Li MH;Wang DS
通讯作者：
Wang DS
影响因子：
16.6
作者：
Y. Takehana;M. Matsuda;T. Myosho;M. Suster;K. Kawakami;T. Shin-I;Y. Kohara;Y. Kuroki;A. Toyoda;A. Fujiyama;S. Hamaguchi;M. Sakaizumi;K. Naruse
通讯作者：
Y. Takehana;M. Matsuda;T. Myosho;M. Suster;K. Kawakami;T. Shin-I;Y. Kohara;Y. Kuroki;A. Toyoda;A. Fujiyama;S. Hamaguchi;M. Sakaizumi;K. Naruse