Improved cellular infiltration into nanofibrous electrospun cross-linked gelatin scaffolds templated with micrometer-sized polyethylene glycol fibers.
Improved cellular infiltration into nanofibrous electrospun cross-linked gelatin scaffolds templated with micrometer-sized polyethylene glycol fibers.
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DOI：
10.1088/1748-6041/6/5/055012
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Subramanian A
中科院分区：
文献类型：
--
作者：
Skotak M;Ragusa J;Gonzalez D;Subramanian A
明胶为基础的纳米纤维支架的平均纤维直径为300 nm的制备与微米级聚乙二醇（PEG）纤维作为牺牲模板。在通过静电纺丝制造支架时，明胶纤维与戊二醛交联，并且使用叔丁醇去除PEG模板以产生孔径范围为10至100 μ m的纳米纤维支架，如用压汞孔隙率法估计的。非模板化明胶基纳米纤维基质的平均孔径为1 µ m。将成纤维细胞接种到两种类型的基于明胶的纳米纤维表面上并培养14天。出于比较的目的，还包括分别具有100 μ m和170 μ m孔径的壳聚糖基和聚氨酯（PU）基大孔支架。使用细胞核染色判断和定量评估作为进入支架的深度的函数的细胞数量。在用牺牲模板和仅明胶纳米纤维支架制备的明胶支架中注意到高达250 μ m和90 μ m的细胞渗透深度。值得注意的是，这里提出的支架制备方案允许即使在高模板含量（95%）的情况下也保持结构完整性，并且可以容易地扩展到用于组织工程的其他类别的电纺聚合物基质。
Gelatin-based nanofibrous scaffolds with a mean fiber diameter of 300 nm were prepared with and without micrometer-sized polyethylene glycol (PEG) fibers that served as sacrificial templates. Upon fabrication of the scaffolds via electrospinning, the gelatin fibers were crosslinked with glutaraldehyde, and the PEG templates were removed using tert-butanol to yield nanofibrous scaffolds with pore diameters ranging from 10 to 100 µm, as estimated with mercury intrusion porosimetry. Non-templated gelatin-based nanofibrous matrices had an average pore size of 1 µm. Fibroblasts were seeded onto both types of the gelatin-based nanfibrous surfaces and cultured for 14 days. For comparative purposes, chitosan-based and polyurethane (PU)-based macroporous scaffolds with pore sizes of 100 µm and 170 µm, respectively, also were included. The number of cells as a function of the depth into the scaffold was judged and quantitatively assessed using nuclei staining. Cell penetration up to a depth of 250 µm and 90 µm was noted in gelatin scaffolds prepared with sacrificial templates and gelatin-only nanofibrous scaffolds. Noticeably, scaffold preparation protocol presented here allowed the structural integrity to be maintained even with high template content (95 %) and can be easily extended towards other classes of electrospun polymer matrices for tissue engineering.
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DOI：
10.1089/ten.tec.2008.0422
发表时间：
2009-09
期刊：
Tissue engineering. Part C, Methods
影响因子：
--
作者：
Saraf A;Lozier G;Haesslein A;Kasper FK;Raphael RM;Baggett LS;Mikos AG
通讯作者：
Mikos AG
影响因子：
10.4
作者：
通讯作者：
--
影响因子：
14
作者：
Bashur, Chris A.;Dahlgren, Linda A.;Goldstein, Aaron S.
通讯作者：
Goldstein, Aaron S.
影响因子：
4.6
作者：
Sundararaghavan, Harini G.;Metter, Robert B.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
6.2
作者：
Skotak, Maciej;Leonov, Alexei P.;Subramanian, Anuradha
通讯作者：
Subramanian, Anuradha