Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus.
Identification of antibody neutralization epitopes on the fusion protein of human metapneumovirus.
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DOI：
10.1099/vir.0.2008/005199-0
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
McCarthy MP
中科院分区：
文献类型：
--
作者：
Ulbrandt ND;Ji H;Patel NK;Barnes AS;Wilson S;Kiener PA;Suzich J;McCarthy MP
人类偏肺病毒（hMPV）与呼吸道合胞病毒（RSV）在遗传上相关;两者都能引起呼吸道疾病，从轻微咳嗽到细支气管炎和肺炎。F蛋白导向的单克隆抗体（mAb）帕利珠单抗已被证明可预防动物和人类的重度下呼吸道RSV感染。我们以前曾报道过一组抗hMPV F蛋白的mAb，它们在体外中和hMPV，在两种情况下，在体内中和hMPV。在这里，我们描述了hMPV单克隆抗体耐药突变体（MARM）的产生，这些中和抗体。对hMPV MARM的F蛋白进行测序鉴定了几个中和表位。有趣的是，定位在hMPV F蛋白上的一些表位与先前定位在RSV F蛋白上的同源区域一致，包括广泛保护性mAb帕利珠单抗所针对的位点。这表明这些同源区域在两种病毒中都发挥着重要的保守功能。
Human metapneumovirus (hMPV) is genetically related to respiratory syncytial virus (RSV); both cause respiratory tract illnesses ranging from a mild cough to bronchiolitis and pneumonia. The F protein-directed monoclonal antibody (mAb) palivizumab has been shown to prevent severe lower respiratory tract RSV infection in animals and humans. We have previously reported on a panel of mAbs against the hMPV F protein that neutralize hMPV in vitro and, in two cases, in vivo. Here we describe the generation of hMPV mAb-resistant mutants (MARMs) to these neutralizing antibodies. Sequencing the F proteins of the hMPV MARMs identified several neutralizing epitopes. Interestingly, some of the epitopes mapped on the hMPV F protein coincide with homologous regions mapped previously on the RSV F protein, including the site against which the broadly protective mAb palivizumab is directed. This suggests that these homologous regions play important, conserved functions in both viruses.
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影响因子：
82.9
作者：
van den Hoogen BG;de Jong JC;Groen J;Kuiken T;de Groot R;Fouchier RA;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
3.7
作者：
Crowe, JE;Firestone, CY;Murphy, BR
通讯作者：
Murphy, BR
影响因子：
2.7
作者：
Corvaisier, C;Bourgeois, C;Pothier, P
通讯作者：
Pothier, P
DOI：
10.1093/protein/15.5.365
发表时间：
2002-05-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Smith, BJ;Lawrence, MC;Colman, PM
通讯作者：
Colman, PM
影响因子：
8
作者：
Null, D;Bimle, C;Top, FH
通讯作者：
Top, FH