A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.
A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.
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DOI：
10.1007/s10544-010-9457-7
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发表时间：
2010-12
影响因子：
2.8
通讯作者：
Lee RT
中科院分区：
文献类型：
--
作者：
Amadi OC;Steinhauser ML;Nishi Y;Chung S;Kamm RD;McMahon AP;Lee RT
微流体技术的出现允许通过用各种生物化学和生物物理刺激来定义局部微环境来控制和询问细胞行为。已经开发了许多方法来产生可溶性因子的梯度，但是这种系统的复杂性或它们不能产生限定的和可控的化学梯度限制了它们的广泛实施。在这里，我们描述了一种新的微流体装置，其采用平行排列的威尔斯和通道，以在源和阱之间的凝胶区域中产生稳定的线性浓度梯度。源和阱威尔斯之间的压力梯度通过与凝胶通道平行的低阻力通道消散，从而使该区域中溶质的对流最小化。我们证明了新设备的能力，以定量在各种类型的细胞的趋化反应，产生一个完整的配置文件的迁移反应，并代表迁移细胞的总数和每个细胞迁移的距离。此外，我们显示了形态发生剂Sonic hedgehog的浓度梯度对3维矩阵中分化神经祖细胞的规格的影响。
The advent of microfluidic technology allows control and interrogation of cell behavior by defining the local microenvironment with an assortment of biochemical and biophysical stimuli. Many approaches have been developed to create gradients of soluble factors, but the complexity of such systems or their inability to create defined and controllable chemical gradients has limited their widespread implementation. Here we describe a new microfluidic device which employs a parallel arrangement of wells and channels to create stable, linear concentration gradients in a gel region between a source and a sink well. Pressure gradients between the source and sink wells are dissipated through low resistance channels in parallel with the gel channel, thus minimizing the convection of solute in this region. We demonstrate the ability of the new device to quantitate chemotactic responses in a variety of cell types, yielding a complete profile of the migratory response and representing the total number of migrating cells and the distance each cell has migrated. Additionally we show the effect of concentration gradients of the morphogen Sonic hedgehog on the specification of differentiating neural progenitors in a 3-dimensional matrix.
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影响因子：
6.1
作者：
Glawdel, Tomasz;Elbuken, Caglar;Ren, Carolyn L.
通讯作者：
Ren, Carolyn L.
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
7.4
作者：
Amarie, Dragos;Glazier, James A.;Jacobson, Stephen C.
通讯作者：
Jacobson, Stephen C.
DOI：
10.3791/271
发表时间：
2007-01-01
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Chung, Bong Geun;Manbachi, Amir;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
成绩：声音刺猬信号传导和神经细胞命运的控制。
影响因子：
11.4
作者：
Briscoe, James
通讯作者：
Briscoe, James