Systematic Screening for Mutant Mouse Lines with Defects in Body Temperature Regulation
Systematic Screening for Mutant Mouse Lines with Defects in Body Temperature Regulation
复制标题
系统筛选体温调节缺陷的突变小鼠系
DOI：
10.1007/978-3-642-28678-0_40
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Klingenspor M
中科院分区：
文献类型：
--
作者：
Willershäuser M;Ehrhardt N;Elvert R;Wirth EK;Schweizer U;Gailus-Durner V;Fuchs H;Hrabě de Angelis M;Rozman J;Klingenspor M
在许多哺乳动物和鸟类中观察到自发的日常麻木以及寒冷和禁食引起的麻木。麻痹的频率和强度在很大程度上受良好表征的环境条件的影响。然而，对体温调节表型变异的遗传成分知之甚少。鉴定影响生理体温调节的突变可能有助于阐明代谢和体温调节行为的新分子机制。因此，我们监测了6个近交系小鼠品系（129/SvJ，AKR/J，C57 BL/6J，C57 BL/6N，Balb/cJ和SWR/J）和146个突变小鼠品系和年龄匹配的对照组的直肠体温（Tb）。在自由采食条件下进行测量，并对食物限制或食物剥夺做出反应。近交系小鼠在自由采食、限食和禁食条件下的Tb2表现出明显的品系特异性差异，雌性小鼠的Tb2平均比雄性小鼠高0.4 ° C。对食物限制的体温调节反应反映了Tb和体重变化之间的权衡，表现出Tb最大下降的小鼠体重损失最少。在自由采食的突变小鼠品系中，146只中有36只的Tb显著高于（15只品系）或低于（21只品系）对照组。在对食物限制和剥夺的反应中，发现了13个Tb降低增强的突变小鼠系，其中两个显示直肠T低于31 ° C。总之，Tbunder自由以及在响应食物剥夺是一个重要的指标，基因型相关的差异对系统功能。根据测试条件，25 - 32%的所有突变株系显示出Tb的显著改变。
Spontaneous daily torpor as well as cold- and fasting-induced torpor is observed in many mammals and birds. The frequency and intensity of torpor are largely influenced by well characterized environmental conditions. The inherited component of phenotypic variation in body temperature regulation is, however, only poorly understood. The identification of mutations affecting physiological body temperature regulation may elucidate novel molecular mechanisms contributing to metabo- and thermoregulatory behavior. Therefore, we monitored rectal body temperature (Tb) in six inbred mouse strains (129/SvJ, AKR/J, C57BL/6J, C57BL/6N, Balb/cJ, and SWR/J) and in 146 mutant mouse lines and age-matched controls. Measurements were taken under ad libitum conditions and in response to either food restriction or food deprivation. Inbred mice showed considerable strain-specific differences inTbunder ad libitum feeding as well as after food restriction and deprivation.Tbwas on average ≈0.4°C higher in females as compared to males in all inbred lines. The thermoregulatory response to food restriction reflected a trade-off between changes inTband body mass with the least body mass loss in mice exhibiting the largest drop inTb. In mutant mouse lines under ad libitum feedingTbof 36 out of 146 was significantly higher (15 lines) or lower (21 lines) as compared with controls. In response to food restriction and deprivation, 13 mutant mouse lines with augmentedTbreduction were found two of which showed rectalTblower than 31°C. In conclusion,Tbunder ad libitum as well as in response to food deprivation is an important indicator for genotype-related differences on systemic functions. Depending on the test conditions 25–32% of all mutant lines showed significant alterations inTb.
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影响因子：
5.8
作者：
Swoap, Steven J.
通讯作者：
Swoap, Steven J.
DOI：
10.1038/oby.2004.188
发表时间：
2004-09-01
期刊：
OBESITY RESEARCH
影响因子：
--
作者：
Meyer, CWE;Klingenspor, M;Heldmaier, G
通讯作者：
Heldmaier, G
肥胖的人体温会低吗？
影响因子：
--
作者：
L. Landsberg;James B. Young;W. Leonard;R. Linsenmeier;F. Turek
通讯作者：
F. Turek
影响因子：
64.8
作者：
TRAYHURN, P;THURLBY, PL;JAMES, WPT
通讯作者：
JAMES, WPT
DOI：
10.1152/ajpgi.00321.2007
发表时间：
2008-03-01
影响因子：
4.5
作者：
Pfluger, Paul T.;Kirchner, Henriette;Tschoep, Matthias H.
通讯作者：
Tschoep, Matthias H.