Measuring the effectiveness of gaseous virus disinfectants.

Measuring the effectiveness of gaseous virus disinfectants.
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DOI:
10.1016/j.biologicals.2015.06.002
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发表时间:
2015-11
期刊:
Biologicals : journal of the International Association of Biological Standardization
影响因子:
--
通讯作者:
Kreil TR
Kreil TR
中科院分区:
其他
文献类型:
--
作者:
Knotzer S;Kindermann J;Modrof J;Kreil TR

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气体消毒的有效性是预防和处理生物技术设施中微生物污染的关键。为了评价气体消毒效果,使用目前可用的载体试验和定制的干雾箱建立了一个缩小的实验室模型。过氧乙酸和过氧化氢(PAA/HP)的混合物进行了研究,例如,在0.4和2.9 mL/m3之间的浓度长达3小时的一组脂质包膜和非脂质包膜病毒的灭活。流感病毒对PAA/HP最敏感,小鼠细小病毒最耐药。牛病毒性腹泻病毒和呼肠孤病毒III表现出中等稳定性和相似的灭活动力学。使用干雾箱避免了将整个实验室用于研究,这使得数据的生成更具成本效益,并允许产生高度可再现的动力学数据。
The efficacy of gaseous disinfection is critical for prevention and treatment of microbial contamination in biotechnological facilities. For an evaluation of gaseous disinfection efficacy, a down-scaled laboratory model was established, using currently available carrier tests and a custom-made dry fog box. A mixture of peroxyacetic acid and hydrogen peroxide (PAA/HP) was investigated as example, at concentrations between 0.4 and 2.9 mL/m3 for up to 3 h for inactivation of a panel of lipid-enveloped and non-lipid-enveloped viruses. The influenza viruses were most sensitive to PAA/HP treatment and minute virus of mice was most resistant. Bovine viral diarrhea virus and reovirus III showed intermediate stability and similar inactivation kinetics. Use of the dry fog box circumvents dedicating an entire lab for the investigation, which renders the generation of data more cost-effective and allows for production of highly reproducible kinetic data.
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