Investigating the Efficacy of a Canine Rabies Vaccine Following Storage Outside of the Cold-Chain in a Passive Cooling Device.
Investigating the Efficacy of a Canine Rabies Vaccine Following Storage Outside of the Cold-Chain in a Passive Cooling Device.
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在被动冷却装置中冷链外部存储后，研究了犬狂犬病疫苗的功效。
DOI：
10.3389/fvets.2021.728271
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发表时间：
2021
影响因子：
3.2
通讯作者：
Lankester F
中科院分区：
文献类型：
--
作者：
Lugelo A;Hampson K;Czupryna A;Bigambo M;McElhinney LM;Marston DA;Kazwala R;Lankester F
背景资料：耐热疫苗大大扩大了消灭天花、脊髓灰质炎和牛瘟等传染病的大规模方案的覆盖面和影响。2015年的一项研究表明，Nobivac®狂犬病疫苗在30°C下实验储存3个月后，其效力不受影响。疫苗在更自然的、波动的温度条件下储存后是否仍然有效仍然未知。我们进行了一项随机对照非劣效性试验，以比较在冷链条件下储存的剂量与在波动温度条件下储存在当地制造的被动冷却装置（“Zeepot”）中的剂量接种后犬的血清学应答。材料和方法：Nobivac®狂犬病疫苗在冷链条件下或Zeepot内储存2个月。每天的环境温度和Zeepot内的温度每3小时记录一次。储存后，412只家犬被随机分配接受冷链或Zeepot储存的Nobivac®狂犬病疫苗。采集基线和疫苗接种后第28天的血样。使用荧光抗体病毒中和试验进行血清学分析，阈值为0.5 IU/ml，以确定血清转化。此外，还检查了犬身体状况评分、性别和年龄对血清转化的影响。结果如下：使用储存在Zeepot内的Nobivac®狂犬病疫苗接种的犬的血清学应答不劣于使用冷链储存疫苗接种的犬的应答（z = 1.1，df = 313，p值= 0.25）。实际上，冷链与非冷链储存的接种后28天组几何平均滴度分别为1.8和2.0 IU/ml。此外，每组中血清转化犬的百分比几乎相同（85%）。身体状况评分（O.R.）2.2，95% CI：1.1-5.1）和血清转化，表明条件差的犬可能对疫苗接种没有预期的反应。结论：我们的研究表明，在Zeepot内升高的波动温度下储存后，Nobivac®狂犬病疫苗的效力不受影响。这些结果对于在低收入和中等收入国家扩大大规模狗疫苗接种计划具有潜在的令人兴奋的应用，特别是对于难以接触到的人群，他们的电力和冷链疫苗储存有限。
Background: Thermostable vaccines greatly improved the reach and impact of large-scale programmes to eliminate infectious diseases such as smallpox, polio, and rinderpest. A study from 2015 demonstrated that the potency of the Nobivac® Rabies vaccine was not impacted following experimental storage at 30°C for 3 months. Whether the vaccine would remain efficacious following storage under more natural, fluctuating temperature conditions remains unknown. We carried out a randomised controlled non-inferiority trial to compare serological responses in dogs following vaccination with doses stored under cold chain conditions with those stored within a locally made Passive Cooling Device (“Zeepot”) under fluctuating temperature conditions. Materials and Methods: Nobivac® Rabies vaccine was stored under either cold-chain conditions or within the Zeepot for 2 months. Daily ambient temperatures and temperatures within the Zeepot were recorded every 3 h. Following storage, 412 domestic dogs were randomly assigned to receive either cold-chain or Zeepot stored Nobivac® Rabies vaccine. Baseline and day 28-post vaccination blood samples were collected. Serological analysis using the Fluorescent Antibody Virus Neutralisation assay was carried out with a threshold of 0.5 IU/ml to determine seroconversion. In addition, the impact of dog Body Condition Score, sex, and age on seroconversion was examined. Results: The serological response of dogs vaccinated using Nobivac® Rabies vaccine stored within the Zeepot was not inferior to the response of dogs vaccinated using cold-chain stored vaccine (z = 1.1, df = 313, p-value = 0.25). Indeed, the 28-day post-vaccination group geometric mean titre was 1.8 and 2.0 IU/ml for cold-chain vs. non-cold-chain storage, respectively. Moreover, the percentage of dogs that seroconverted in each arm was almost identical (85%). There was a positive linear trend between Body Condition Score (O.R. 2.2, 95% CI: 1.1–5.1) and seroconversion, suggesting dogs of poor condition may not respond as expected to vaccination. Conclusions: Our study demonstrated the potency of Nobivac® Rabies vaccine is not impacted following storage under elevated fluctuating temperatures within a Zeepot. These results have potentially exciting applications for scaling up mass dog vaccination programmes in low-and-middle income countries, particularly for hard-to-reach populations with limited access to power and cold-chain vaccine storage.
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影响因子：
3.8
作者：
Hampson K;Coudeville L;Lembo T;Sambo M;Kieffer A;Attlan M;Barrat J;Blanton JD;Briggs DJ;Cleaveland S;Costa P;Freuling CM;Hiby E;Knopf L;Leanes F;Meslin FX;Metlin A;Miranda ME;Müller T;Nel LH;Recuenco S;Rupprecht CE;Schumacher C;Taylor L;Vigilato MA;Zinsstag J;Dushoff J;Global Alliance for Rabies Control Partners for Rabies Prevention
通讯作者：
Global Alliance for Rabies Control Partners for Rabies Prevention
影响因子：
5.5
作者：
Kennedy, Lorna J.;Lunt, Mark;Ollier, William E. R.
通讯作者：
Ollier, William E. R.
影响因子：
2.1
作者：
Berndtsson LT;Nyman AK;Rivera E;Klingeborn B
通讯作者：
Klingeborn B
影响因子：
2.6
作者：
Wambura, PN;Kapaga, AM;Hyera, JMK
通讯作者：
Hyera, JMK
影响因子：
2.9
作者：
Lugelo A;Hampson K;Bigambo M;Kazwala R;Lankester F
通讯作者：
Lankester F