High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow.
High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow.
复制标题
高脂饮食导致牙龈血流量减少的代谢紊乱机制。
DOI：
10.3389/fphys.2021.625780
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发表时间：
2021
影响因子：
4
通讯作者：
Tyagi SC
中科院分区：
文献类型：
--
作者：
Stanisic D;Jeremic N;Majumder S;Pushpakumar S;George A;Singh M;Tyagi SC
肠道微生物组在人类健康中具有非常重要的作用，其对许多疾病发展的影响是众所周知的。在这项研究中，我们调查了高脂饮食（HFD）的影响，对发病的生态失调，牙龈血流量减少，牙周基质重塑。建立了HFD组和Lactobacillus rhamnosus GG（LGG）益生菌模型，并进行了12周的实验研究。在小鼠处死前24小时收集粪便样品，随后进行短链脂肪酸（SCFA）分析、DNA提取和用于宏基因组分析的测序。在处死动物后，我们收集牙周组织并进行全面的形态学和遗传学分析。虽然HFD减少拟杆菌、SCFA和牙龈血流量，但这种类型的饮食增加了厚壁菌门、脂多糖（LPS）结合蛋白、TLR 4、促炎细胞因子（TNF-α、IL-1β和IL-6）、基质金属蛋白酶（MMP-2和MMP-9）表达，还改变了骨吸收标志物（OPG和RANKL）。然而，LGG治疗减轻了这些影响。因此，观察到HFD通过炎症、基质降解和功能重塑增加分子重塑，并因此导致牙龈血流量减少。所有这些变化都可能导致牙槽骨的丢失和牙周病的发展。
The gut microbiome has a very important role in human health and its influence on the development of numerous diseases is well known. In this study, we investigated the effect of high fat diet (HFD) on the onset of dysbiosis, gingival blood flow decreases, and the periodontal matrix remodeling. We established a dysbiosis model (HFD group) and probiotic model by Lactobacillus rhamnosus GG (LGG) treatment for 12weeks. Fecal samples were collected 24h before mice sacrificing, while short chain fatty acids (SCFA) analysis, DNA extraction, and sequencing for metagenomic analysis were performed afterwards. After sacrificing the animals, we collected periodontal tissues and conducted comprehensive morphological and genetic analyses. While HFD reduced Bacteroidetes, SCFA, and gingival blood flow, this type of diet increased Firmicutes, lipopolysaccharide (LPS) binding protein, TLR4, pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), matrix metalloproteinases (MMP-2 and MMP-9) expression, and also altered markers of bone resorption (OPG and RANKL). However, LGG treatment mitigated these effects. Thus, it was observed that HFD increased molecular remodeling via inflammation, matrix degradation, and functional remodeling and consequently cause reduced gingival blood flow. All of these changes may lead to the alveolar bone loss and the development of periodontal disease.
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影响因子：
3
作者：
BAAB, DA;OBERG, PA
通讯作者：
OBERG, PA
DOI：
10.1016/j.pathophys.2019.06.004
发表时间：
2019-09-01
期刊：
Pathophysiology : the official journal of the International Society for Pathophysiology
影响因子：
--
作者：
Becker, F;Gavins, F N E;Alexander, J S
通讯作者：
Alexander, J S
影响因子：
6.7
作者：
Gatej, Simona M.;Marino, Victor;Bartold, Peter M.
通讯作者：
Bartold, Peter M.
影响因子：
--
作者：
Barreiros, Driely;Nelson Filho, Paulo;Silva, Raquel Assed Bezerra
通讯作者：
Silva, Raquel Assed Bezerra
影响因子：
5.6
作者：
Bai Y;Wei Y;Wu L;Wei J;Wang X;Bai Y
通讯作者：
Bai Y