Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry.
Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry.
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DOI：
10.1016/j.nano.2017.11.020
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Luchini A
中科院分区：
文献类型：
--
作者：
Steinberg HE;Russo P;Angulo N;Ynocente R;Montoya C;Diestra A;Ferradas C;Schiaffino F;Florentini E;Jimenez J;Calderón M;Carruthers VB;Gilman RH;Liotta L;Luchini A
诊断临床弓形虫病仍然是一个挑战，因此限制了人类临床样本的可用性。尽管鼠模型是人类反应的近似值，但它们的确定感染状态和组织的可用性使其对诊断发展过程至关重要。水凝胶网纳米颗粒用于将抗原浓缩以检测到质谱的可检测水平。使用七个T. gondii分离株来开发一组潜在的肽序列，以通过平行反应监测（PRM）质谱法检测。将纳米颗粒与降低的速度裂解液浓度一起孵育，以探索PRM检测的极限。通过定量实时PCR证实其弓形虫病感染状态的小鼠在水凝胶网状浓度后通过PRM测试了已知的T. gondii肽。 GRA1，GRA12，ROP4，ROP5，SAG1和SAG2A蛋白的肽在尿液的纳米颗粒浓度后通过PRM检测，确认在感染小鼠的尿液中检测T. gondii抗原。
Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability makes them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven T. gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.
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影响因子：
2.5
作者：
Luchini A;Fredolini C;Espina BH;Meani F;Reeder A;Rucker S;Petricoin EF 3rd;Liotta LA
通讯作者：
Liotta LA
影响因子：
7.4
作者：
Magni R;Espina BH;Shah K;Lepene B;Mayuga C;Douglas TA;Espina V;Rucker S;Dunlap R;Petricoin EF;Kilavos MF;Poretz DM;Irwin GR;Shor SM;Liotta LA;Luchini A
通讯作者：
Luchini A
影响因子：
2.6
作者：
Dubey, JP
通讯作者：
Dubey, JP
影响因子：
2.1
作者：
Daryani, Ahmad;Sharif, Mehdi;Rasouli, Mehdi
通讯作者：
Rasouli, Mehdi
DOI：
10.1111/j.1550-7408.1992.tb04844.x
发表时间：
1992-07-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
SAFFER, LD;MERCEREAUPUIJALON, O;SCHWARTZMAN, JD
通讯作者：
SCHWARTZMAN, JD