Umbilical cord Wharton's jelly repeated culture system: a new device and method for obtaining abundant mesenchymal stem cells for bone tissue engineering.
Umbilical cord Wharton's jelly repeated culture system: a new device and method for obtaining abundant mesenchymal stem cells for bone tissue engineering.
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脐带沃顿氏果冻重复培养系统：一种获得丰富的间充质干细胞用于骨组织工程的新装置和方法
DOI：
10.1371/journal.pone.0110764
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Chang Z;Hou T;Xing J;Wu X;Jin H;Li Z;Deng M;Xie Z;Xu J
迄今为止，用于接种再生支架的各种类型的细胞已被用于骨组织工程。在种子细胞中，来源于人脐带华顿氏胶的间充质干细胞（hUCMSCs）代表了一个有前途的候选者，并由于缺乏伦理争议、可获得性、来源于供体的非侵入性程序、污染风险降低、成骨分化能力和更高的免疫调节能力而具有骨组织工程的潜力。然而，目前的培养方法有些复杂和效率低下，往往不能充分利用脐带（UC）组织。此外，这些培养过程不能在大规模和严格的质量控制下进行。因此，使用目前的培养方法只能收获少量细胞。为了解决这些问题，我们设计并评估了一个UC沃顿的果冻重复培养装置。利用该装置，从重复培养中获得hUCMSCs，并比较其数量和生物学特性。我们发现，使用我们的培养装置，将所有组织块保留在培养皿底部，获得的细胞总数增加了15-20倍，并且减少了原代所需的时间。此外，从重复培养物中收获的细胞在其免疫表型、多向分化潜能或增殖、骨诱导能力和最终成骨方面没有表现出显著差异。重复培养架的应用不仅充分利用了华顿氏胶，而且简化和规范了培养过程，显著提高了培养效率。总之，使用RCF可以获得大量质量可靠的hUCMSCs。
To date, various types of cells for seeding regenerative scaffolds have been used for bone tissue engineering. Among seed cells, the mesenchymal stem cells derived from human umbilical cord Wharton’s jelly (hUCMSCs) represent a promising candidate and hold potential for bone tissue engineering due to the the lack of ethical controversies, accessibility, sourced by non-invasive procedures for donors, a reduced risk of contamination, osteogenic differentiation capacities, and higher immunomodulatory capacity. However, the current culture methods are somewhat complicated and inefficient and often fail to make the best use of the umbilical cord (UC) tissues. Moreover, these culture processes cannot be performed on a large scale and under strict quality control. As a result, only a small quantity of cells can be harvested using the current culture methods. To solve these problems, we designed and evaluated an UC Wharton’s jelly repeated culture device. Using this device, hUCMSCs were obtained from the repeated cultures and their quantities and biological characteristics were compared. We found that using our culture device, which retained all tissue blocks on the bottom of the dish, the total number of obtained cells increased 15–20 times, and the time required for the primary passage was reduced. Moreover, cells harvested from the repeated cultures exhibited no significant difference in their immunophenotype, potential for multilineage differentiation, or proliferative, osteoinductive capacities, and final osteogenesis. The application of the repeated culture frame (RCF) not only made full use of the Wharton’s jelly but also simplified and specified the culture process, and thus, the culture efficiency was significantly improved. In summary, abundant hUCMSCs of dependable quality can be acquired using the RCF.
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影响因子：
5.2
作者：
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通讯作者：
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