Mechanistic examination of protein release from polymer nanofibers.
Mechanistic examination of protein release from polymer nanofibers.
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DOI：
10.1021/mp800160p
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发表时间：
2009-03
影响因子：
4.9
通讯作者：
Gemeinhart RA
中科院分区：
文献类型：
--
作者：
Gandhi M;Srikar R;Yarin AL;Megaridis CM;Gemeinhart RA
在过去的几十年里，治疗性蛋白质已经成为一类重要的药物制剂。蛋白质的效力、快速消除和全身副作用促使蛋白质时空控制释放的需求可能比其他任何活性治疗分子都更大。这项工作研究了两种模型蛋白质化合物牛血清白蛋白(BSA)和抗整合素抗体(AI)从电纺聚己内酯(PCL)纳米纤维垫中的释放。抗整合素抗体被选为抗体治疗的模型，尤其是抗整合素抗体是一类很有前途的治疗癌症和血管生成疾病的分子。释放动力学被实验研究，并在最近发表的从非降解-或非常缓慢降解的纤维中解吸-限制药物释放的理论的框架内进行解释。结果与蛋白质从聚合物表面解吸为主的释放机制一致，而聚己内酯纳米纤维没有明显的降解速率。
Therapeutic proteins have emerged as a significant class of pharmaceutical agents over the past several decades. The potency, rapid elimination, and systemic side-effects have prompted the need of spatiotemporally controlled release for proteins maybe more than any other active therapeutic molecules. This work examines the release of two model protein compounds, bovine serum albumin (BSA) and an anti-integrin antibody (AI), from electrospun polycaprolactone (PCL) nanofiber mats. The anti-integrin antibody was chosen as a model of antibody therapy; in particular, anti-integrin antibodies are a promising class of therapeutic molecules for cancer and angiogenic diseases. The release kinetics were studied experimentally and interpreted in the framework of a recently published theory of desorption-limited drug release from non-degrading—or very slowly degrading—fibers. The results are consistent with a protein release mechanism dominated by desorption from the polymer surface, while the polycaprolactone nanofibers are not degrading at an appreciable rate.
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影响因子：
6.2
作者：
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通讯作者：
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DOI：
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发表时间：
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